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Title

EVALUATION OF EXCRETORY-SECRETORY PROTOSCOLICES , GERMINAL LAYER AND HYDATID FLUID ANTIGENS FOR DIAGNOSIS OF HYDATIDOSIS

Pages

  339-348

Abstract

 Echinococcus granulosus is caused hydatidosis as a zoonotic diseases in human and animal. Infected liver and lungs of sheep with HYDATID CYST were gathered from Ahvaz slaughterhouse and HYDATID CYST fluid, protoscolexes, and germinal layers of cysts were separated and cultured in RPMI strile medium. In this study, evaluation of the reaction of the antigen of the cyst fluid, germinal layer and protoscolices in the presence of serum prepared from sheep and mice was carried out and the blood required for preparation of infected and uninfected sera was prepared from 100 sheep during slaughtering. The counterimmunoelectrophoresis test was evaluated to HYDATID CYST diagnosis. In 50 samples of serum infected with HYDATID CYST, by counterimmunoelectrophoresis method, 80% of the sera using the antigen of the hydatid  fluid, 72% using the antigen of the protoscolexes ES., and 92% using the antigen of the germinal layer ES. were showed positive reaction, and in 5 samples of sera infected with HYDATID CYST in mice, by counterimmunoelectrophoresis method, 80% of sera using the HYDATID CYST fluid, 80% using the antigen of protoscolexes ES, and 100% using the anigen of germinal layer ES showed positive reaction. According to the findings of this study, the antigen of germinal layer, ES having a higher percentage of positive reaction, is more applicable for obtaining a better result from this test. The advantages of this method must be compared with other methods.

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    APA: Copy

    RAZI JALALI, MOHAMMAD HOSSEIN, ALBORZI, H., GHORBANPOOR, M., & AGABEYGI, A.. (2018). EVALUATION OF EXCRETORY-SECRETORY PROTOSCOLICES , GERMINAL LAYER AND HYDATID FLUID ANTIGENS FOR DIAGNOSIS OF HYDATIDOSIS. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), 11(4 (44) ), 339-348. SID. https://sid.ir/paper/183018/en

    Vancouver: Copy

    RAZI JALALI MOHAMMAD HOSSEIN, ALBORZI H., GHORBANPOOR M., AGABEYGI A.. EVALUATION OF EXCRETORY-SECRETORY PROTOSCOLICES , GERMINAL LAYER AND HYDATID FLUID ANTIGENS FOR DIAGNOSIS OF HYDATIDOSIS. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)[Internet]. 2018;11(4 (44) ):339-348. Available from: https://sid.ir/paper/183018/en

    IEEE: Copy

    MOHAMMAD HOSSEIN RAZI JALALI, H. ALBORZI, M. GHORBANPOOR, and A. AGABEYGI, “EVALUATION OF EXCRETORY-SECRETORY PROTOSCOLICES , GERMINAL LAYER AND HYDATID FLUID ANTIGENS FOR DIAGNOSIS OF HYDATIDOSIS,” VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), vol. 11, no. 4 (44) , pp. 339–348, 2018, [Online]. Available: https://sid.ir/paper/183018/en

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