IDENTIFICATION OF SCID CARRIERS AMONG IRANIAN ARAB HORSES USING A TEST BASED ON PCR FOR DNA-PKCS GENE

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SEYEDABADI H.; AFRAZ F.; AMIRINIA S.; BANABAZI M.H.; VASEJI N.

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Journal: ANIMAL SCIENCE AND RESEARCH JOURNAL Year:2008 | Volume:1 | Issue:1 Page(s): 43-48

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Title

IDENTIFICATION OF SCID CARRIERS AMONG IRANIAN ARAB HORSES USING A TEST BASED ON PCR FOR DNA-PKCS GENE

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Keywords

SCIDQ3

DNA-PKCS GENEQ3

IRANIAN ARAB HORSEQ3

PCRQ2

Abstract

Severe Combined Immunodeficiency Disease (SCID) of horses was appeared as a deficiency of T and B lymphocytes in foals and resulted in death, caused by infection with opportunistic pathogens, by 5 month of age. This disease is a genetic autosomal recessive trait. A 5-basepair deletion in the horse gene encoding DNA-protein kinase catalytic subunit (DNA-PKCS) was discovered which is responsible for SCID in Arabian horses. We have developed a PCR-based test for detection of the gene defect from 5-basepair deletion in DNA-PKCS GENE. The carriers are heterozygote (259 and 254 bp bands) and dominant and recessive homozygotes have 259bp and 254bp bands, respectively. A total 120 Arab horse were tested and all were homozygote for 259 bp that is noncarrier. In conclusion, we suggest further study on Arab horses throughout of Iran to finding any probable carriers.

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APA: Copy

SEYEDABADI, H., AFRAZ, F., AMIRINIA, S., BANABAZI, M.H., & VASEJI, N.. (2008). IDENTIFICATION OF SCID CARRIERS AMONG IRANIAN ARAB HORSES USING A TEST BASED ON PCR FOR DNA-PKCS GENE. ANIMAL SCIENCE AND RESEARCH JOURNAL, 1(1), 43-48. SID. https://sid.ir/paper/186532/en

Vancouver: Copy

SEYEDABADI H., AFRAZ F., AMIRINIA S., BANABAZI M.H., VASEJI N.. IDENTIFICATION OF SCID CARRIERS AMONG IRANIAN ARAB HORSES USING A TEST BASED ON PCR FOR DNA-PKCS GENE. ANIMAL SCIENCE AND RESEARCH JOURNAL[Internet]. 2008;1(1):43-48. Available from: https://sid.ir/paper/186532/en

IEEE: Copy

H. SEYEDABADI, F. AFRAZ, S. AMIRINIA, M.H. BANABAZI, and N. VASEJI, “IDENTIFICATION OF SCID CARRIERS AMONG IRANIAN ARAB HORSES USING A TEST BASED ON PCR FOR DNA-PKCS GENE,” ANIMAL SCIENCE AND RESEARCH JOURNAL, vol. 1, no. 1, pp. 43–48, 2008, [Online]. Available: https://sid.ir/paper/186532/en

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