The ggenetic diversity of some citrus varieties using microsatellite molecular markers

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BABAKHANI B.; NAGHASHI Y.; HOSSEINI BOLDAJI S.A.; HOSHANI M.

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Journal: PLANT ECOPHYSIOLOGY (ARSANJAN BRANCH) Year:2020 | Volume:12 | Issue:41 Page(s): 271-280

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Title

The ggenetic diversity of some citrus varieties using microsatellite molecular markers

Author(s)

BABAKHANI B. | NAGHASHI Y. | HOSSEINI BOLDAJI S.A. | HOSHANI M. | Issue Writer Certificate

Keywords

CitrusQ2

marker ISSRQ2

genetic diversityQ2

categoryQ2

Abstract

The genetic diversity of 29 varieties of Citrus, including varieties of orange, mandarin, bergamot, pomelo and natural types through a variety of short repetitive sequences using 8 microsatellite markers were evaluated. Genomic DNA extracted from the samples using the amplification and products using polyacrylamide gel electrophoresis instruments were Vasrshth. A total of 97 bands were scored for marker ISSR, 78 bands were polymorphic bands of 22/80 percent. The highest and lowest percentage of polymorphic ISSR-8 primers respectively with 90% and 73% polymorphic ISSR-5, respectively. The average value of PIC was 18/0 in this test. Most of the PIC into primer ISSR-6 and ISSR-8 with 27/0 and lowest primer ISSR-1 with 12/0 respectively. Study dendrogram of cluster analysis UPGMA method with simple matching coefficient, cultivars classified into five main groups. Pomelo in our study were in a separate cluster. ISSR molecular marker Satsuma mandarin and clementine Nvls Suji Yama were grouped into two distinct groups. Views about the use of ISSR markers to differentiate genotypes indicate that the indicator is able to differentiate between genotypes are very close and with the fewest mistakes possible genotypes evaluated each figure.

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APA: Copy

BABAKHANI, B., NAGHASHI, Y., HOSSEINI BOLDAJI, S.A., & HOSHANI, M.. (2020). The ggenetic diversity of some citrus varieties using microsatellite molecular markers. PLANT ECOPHYSIOLOGY (ARSANJAN BRANCH), 12(41 ), 271-280. SID. https://sid.ir/paper/188449/en

Vancouver: Copy

BABAKHANI B., NAGHASHI Y., HOSSEINI BOLDAJI S.A., HOSHANI M.. The ggenetic diversity of some citrus varieties using microsatellite molecular markers. PLANT ECOPHYSIOLOGY (ARSANJAN BRANCH)[Internet]. 2020;12(41 ):271-280. Available from: https://sid.ir/paper/188449/en

IEEE: Copy

B. BABAKHANI, Y. NAGHASHI, S.A. HOSSEINI BOLDAJI, and M. HOSHANI, “The ggenetic diversity of some citrus varieties using microsatellite molecular markers,” PLANT ECOPHYSIOLOGY (ARSANJAN BRANCH), vol. 12, no. 41 , pp. 271–280, 2020, [Online]. Available: https://sid.ir/paper/188449/en

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