The effect of addition of different levels of permeable and non-permeable cryoprotectants on freezing capacity of stallions sperm

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VAFAEI FARHAD; ZARE SHAHNEH AHMAD; Kohram Hmid; SEIFI JAMADI AFSHIN

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Journal: Iranian Journal of Animal Science Year:2018 | Volume:49 | Issue:1 Page(s): 27-32

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Title

The effect of addition of different levels of permeable and non-permeable cryoprotectants on freezing capacity of stallions sperm

Author(s)

VAFAEI FARHAD | ZARE SHAHNEH AHMAD | Kohram Hmid | SEIFI JAMADI AFSHIN | Issue Writer Certificate

Keywords

CASAQ1

cryoprotectantQ2

extenderQ1

spermQ1

stallionQ1

Abstract

The aim of this study was to evaluate the effect of addition of three different permeable (Glycerol, Dimethyl formamide and Dimethyl acetamid) and two different non-permeable cryoprotectant (Trehalose and sucrose) to the extender on freezing capacity of Turkmen stallions’ sperm. Four healthy mature Turkmen stallions (8-10 years old) were used. Collected semen from stallions were processed and pooled after gel removal and before allocating to treatments [glycerol, dimethyl formamide and dimethyl acetamid (5 or 7 percent) and trehalose or sucrose (50 and 100 mM)]. The prepared treatments were frozen according to a standard method. After thawing viability and motility of all treatments were accessed. The results showed that the viability and motility of spermatozoa treated with extender containing 5 or 7 % dimethyl formamide (68. 6 ± 0. 93 and 63. 2 ± 2. 22 respectively) or dimethyl acetamid (68. 6 ± 1. 69 and 63. 6 ± 2. 76 respectively) were significantly higher than those which treated with 5 or 7 % glycerol (56. 8 ± 3. 06 and 55. 6 ± 1. 03 respectively) (P<0. 05). Also, results showed that the viability and motility of stallion spermatozoa were not influenced by the non-permeable cryoprotectants (P<0. 05). We concluded that dimethyl acetamid and dimethyl formamide can be proper alternatives for glycerol as a common stallion spermatozoa cryoprotectant and the sucrose and trehalose had no significant effect on the stallion sperm quality after freeze-thawing process.

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APA: Copy

VAFAEI, FARHAD, ZARE SHAHNEH, AHMAD, Kohram, Hmid, & SEIFI JAMADI, AFSHIN. (2018). The effect of addition of different levels of permeable and non-permeable cryoprotectants on freezing capacity of stallions sperm. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 49(1 ), 27-32. SID. https://sid.ir/paper/193522/en

Vancouver: Copy

VAFAEI FARHAD, ZARE SHAHNEH AHMAD, Kohram Hmid, SEIFI JAMADI AFSHIN. The effect of addition of different levels of permeable and non-permeable cryoprotectants on freezing capacity of stallions sperm. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2018;49(1 ):27-32. Available from: https://sid.ir/paper/193522/en

IEEE: Copy

FARHAD VAFAEI, AHMAD ZARE SHAHNEH, Hmid Kohram, and AFSHIN SEIFI JAMADI, “The effect of addition of different levels of permeable and non-permeable cryoprotectants on freezing capacity of stallions sperm,” IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 49, no. 1 , pp. 27–32, 2018, [Online]. Available: https://sid.ir/paper/193522/en

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