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Information Journal Paper

Title

The effect of intra amniotic injection of zinc-methionine and nano zinc-methionine on metallothionein gene expression in the broiler chickens

Author(s)

Razani Kolsoom | MOTTAGHITALAB MAJID | Heseini Moghaddam Seyed Hosein | Issue Writer Certificate 

Pages

  621-632

Abstract

 The effects of intra amniotic injection of zinc-methionine and nano zinc-methionine on metallothionein mRNA expression of chicken’ s liver and intestine were studied. Four hundred fertiled eggs (Ross 308 strain) were allocated to 4 treatments, 4 replicates/ treatment and 25 eggs per replicate using a CRD (Complete Random Design) design. Experimental treatments were: 1-no injection (negative control), 2-injection of 1 ml 0. 9% saline solution (positive control), 3-injection of 1 ml 25% zinc-methionine solution and 4-injection of 1 ml 25% nano-zinc-methionine solution. On day 17 of incubation, eggs were injected using insulin syringes. Results showed that in Ovo injection lead to no significant effect on hatchability. The injection of zinc-methionine and nano zinc-methionine caused significant (P<0. 01) increase in chicks weight at hatch, and a week after that. A similar result was obtained for liver and intestine samples. Groups treated with zinc-methionine and nano zinc-methionine showed significant (P<0. 01) increase in metallothionein mRNA expression in liver and intestine and remained significant (P<0. 01) at day 7 after hatch. Experimental treatments significantly (P<0. 01) increased the liver and intestine metallothionein content at day 1 and 7 after hatch. Conclusion is that in ovo injection of zinc-methionine and nano zinc-methionine lead to increase body weight at first 3weeks of Broiler rearing.

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    APA: Copy

    Razani, Kolsoom, MOTTAGHITALAB, MAJID, & Heseini Moghaddam, Seyed Hosein. (2017). The effect of intra amniotic injection of zinc-methionine and nano zinc-methionine on metallothionein gene expression in the broiler chickens. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 47(4 ), 621-632. SID. https://sid.ir/paper/193651/en

    Vancouver: Copy

    Razani Kolsoom, MOTTAGHITALAB MAJID, Heseini Moghaddam Seyed Hosein. The effect of intra amniotic injection of zinc-methionine and nano zinc-methionine on metallothionein gene expression in the broiler chickens. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2017;47(4 ):621-632. Available from: https://sid.ir/paper/193651/en

    IEEE: Copy

    Kolsoom Razani, MAJID MOTTAGHITALAB, and Seyed Hosein Heseini Moghaddam, “The effect of intra amniotic injection of zinc-methionine and nano zinc-methionine on metallothionein gene expression in the broiler chickens,” IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 47, no. 4 , pp. 621–632, 2017, [Online]. Available: https://sid.ir/paper/193651/en

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