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Information Journal Paper

Title

DETERMINATION OF PHOSPHOLIPASE A2 ACTIVITY IN THE VENOM OF IRANIAN SNAKES

Pages

  23-25

Keywords

Abstract

 Phospholipas A2 (ph A2) which is present in the VENOM of many SNAKEs ego elapids, viperids, crotalids, seasnakes and even cloubrids, hydrolyses phospholipids in cell membrane and consequently lyses the red blood cells. The aim of this study is to find a rapid, sensitive and useful method to determine the activity of this enzyme in VENOM.The procedure is to take 0.1 ml of each VENOM (stock cone. 100 mg/ ml) and add it to suspension of egg yolk. After the incubation, the suspension was read in a spectrophotometer model U V- 160 A shimadzu (Japan) at 925 nm against Nacl as blank. The difference between blank absorbance and VENOM absorbance was calculated as enzyme activity. The results of above experiments showed the following activites, Naja naja oxiana 525, Vipera lebetina 438, Vipera albicornuta 712, Pseudocerastes persicus 750, Echis carinatus 730, Aghistrodon halys 470 (U/mg). Our results showed the highest activity was belonging to P. persicus and the lowest belong to V.lebetina, the comparison of the enzyme activity in IRANian VENOMs with foreign VENOMs showed higher activity for IRANian VENOMs.

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    APA: Copy

    TOUFANI, M., AMINIAN, M., AKBARI, A., & TABATABAEI RAD, S.M.. (2003). DETERMINATION OF PHOSPHOLIPASE A2 ACTIVITY IN THE VENOM OF IRANIAN SNAKES. PAJOUHESH-VA-SAZANDEGI, 16(1 (58 IN ANIMAL AND FISHERIES SCIENCES)), 23-25. SID. https://sid.ir/paper/19511/en

    Vancouver: Copy

    TOUFANI M., AMINIAN M., AKBARI A., TABATABAEI RAD S.M.. DETERMINATION OF PHOSPHOLIPASE A2 ACTIVITY IN THE VENOM OF IRANIAN SNAKES. PAJOUHESH-VA-SAZANDEGI[Internet]. 2003;16(1 (58 IN ANIMAL AND FISHERIES SCIENCES)):23-25. Available from: https://sid.ir/paper/19511/en

    IEEE: Copy

    M. TOUFANI, M. AMINIAN, A. AKBARI, and S.M. TABATABAEI RAD, “DETERMINATION OF PHOSPHOLIPASE A2 ACTIVITY IN THE VENOM OF IRANIAN SNAKES,” PAJOUHESH-VA-SAZANDEGI, vol. 16, no. 1 (58 IN ANIMAL AND FISHERIES SCIENCES), pp. 23–25, 2003, [Online]. Available: https://sid.ir/paper/19511/en

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    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
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