EVALUATION OF THREE MICROSATELLITE MARKERS OARFCB3O4, OARAE64 AND MAF64 IN LORI-BAKHTIARI STRAIN SHEEP

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DOOSTI A.; DAYANINIA M.; MOSHKELANI S.

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Journal: JOURNAL OF VETERINARY PATHOBIOLOGY Year:2009 | Volume:1 | Issue:1 Page(s): 49-52

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Title

EVALUATION OF THREE MICROSATELLITE MARKERS OARFCB3O4, OARAE64 AND MAF64 IN LORI-BAKHTIARI STRAIN SHEEP

Author(s)

DOOSTI A. | DAYANINIA M. | MOSHKELANI S. | Issue Writer Certificate

Keywords

LORI-BAKHTIARI SHEEPQ2

GENETIC MARKERSQ3

MICROSATELLITESQ2

Abstract

Nowadays with the development of molecular genetic techniques, animal identification and parentage testing is possible using a lot of molecular markers. MICROSATELLITES are simple sequences of DNA consisting of short tandem repeats and according to the size have a high polymorphism, and for this reason, they are used as tools for recognizing the identity and also in the study of species, but by now, any special function have not been known for them in genome level. The aim of this study was to examine the presence of three microsatellite markers OarAE64, OarFCB3O4 and MAF64 in the genome of Lori-Bakhtiari strain sheep and to determine polymorphism in this species. Blood samples collected from 57 Lori-Bakhtiari strain sheep and DNA was extracted by modify salting out procedure. The polymerase chain reaction was used for amplification of these markers with the specific primers and The PCR products were resolved on a non-denaturing 10% polyacrylamide gel by electrophoresis. For OarFCB3O4 marker, four different sizes were seen at the ranges of 130-170 bp. Also, for two markers including OarAE64 and MAF64, 120-150 bp and 130-150 bp DNA fragments, were obtained, respectively. Since that those markers in all of the studying sheep, have various kinds of DNA fragments. Consequently it seems that these three markers can be used as an applicable marker for identifying Lori-l3akhtiari sheep.

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APA: Copy

DOOSTI, A., DAYANINIA, M., & MOSHKELANI, S.. (2009). EVALUATION OF THREE MICROSATELLITE MARKERS OARFCB3O4, OARAE64 AND MAF64 IN LORI-BAKHTIARI STRAIN SHEEP. JOURNAL OF VETERINARY PATHOBIOLOGY, 1(1), 49-52. SID. https://sid.ir/paper/195537/en

Vancouver: Copy

DOOSTI A., DAYANINIA M., MOSHKELANI S.. EVALUATION OF THREE MICROSATELLITE MARKERS OARFCB3O4, OARAE64 AND MAF64 IN LORI-BAKHTIARI STRAIN SHEEP. JOURNAL OF VETERINARY PATHOBIOLOGY[Internet]. 2009;1(1):49-52. Available from: https://sid.ir/paper/195537/en

IEEE: Copy

A. DOOSTI, M. DAYANINIA, and S. MOSHKELANI, “EVALUATION OF THREE MICROSATELLITE MARKERS OARFCB3O4, OARAE64 AND MAF64 IN LORI-BAKHTIARI STRAIN SHEEP,” JOURNAL OF VETERINARY PATHOBIOLOGY, vol. 1, no. 1, pp. 49–52, 2009, [Online]. Available: https://sid.ir/paper/195537/en

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