DIAGNOSIS AND DETECTION OF MYCOPLASMA SYNOVIAE FROM COMMERCIAL POULTRY FLOCKS USING POLYMERAS CHAIN REACTION (PCR) BASED ON AMPLIFICATION OF VLHA GENE

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MAGHAMI M.; POURBAKHSH S.A.; HOMAYOUNIMEHR A.R.; MOHAJERANI H.R.; ASHTARI A.; BAYATZADEH M.A.

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Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2013 | Volume:9 (NEW) | Issue:4 (39) Page(s): 837-845

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Title

DIAGNOSIS AND DETECTION OF MYCOPLASMA SYNOVIAE FROM COMMERCIAL POULTRY FLOCKS USING POLYMERAS CHAIN REACTION (PCR) BASED ON AMPLIFICATION OF VLHA GENE

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Keywords

MYCOPLASMA SYNOVIAEQ2

VARIABLE LIPOPROTEIN HEAMAGGLUTININQ2

VLHA GENEQ2

PCRQ2

ISOLATIONQ2

DIAGNOSISQ2

DETECTIONQ2

Abstract

MYCOPLASMA SYNOVIAE is a major avian pathogen and cases many economic losses on poultry industry in Iran, MYCOPLASMA SYNOVIAE synthesizes VARIABLE LIPOPROTEIN HEAMAGGLUTININ (VlhA), is believed to play a major in pathogenesis of the disease. The objective of the present study was to DIAGNOSIS and DETECTION of MYCOPLASMA SYNOVIAE isolates based on the VLHA GENE. This study was designed to detect MS through culture ISOLATION and polymerase chain reaction (PCR) assay to demonstrated the involvement of MS infection in trachea and the lung/air sac samples taken from commercial poultry flocks of provinces: Tehran, Qazvin and Markazi with clinical signs of the disease. Moreover the specific primers to the Mycoplasma genuse from the primes complementary to the single-copy conserved 5’ end of VLHA GENE were used for DETECTION of MS by vlhA-PCR. PCR results, in addition to identification of Mycoplasma specie, revealed variable sizes of 350-400bp for each isolates. Of the 43 swabs 28 (65%) yielded one of the potentially pathogenic Mycoplasmas evaluated for using PPLO agar culture diagnostic method, and 33 (76%) yielded one of the potentially pathogenic Mycoplasmas evaluated for Mycoplasma genuse PCR as diagnostic metod and 24 (55%) swabs yielded MYCOPLASMA SYNOVIAE for using vlhA PCR as diagnostic metod. The results of this study demonstrated that PCR based on amplification of conserved 5́ end of VLHA GENE is an efficient tool for DETECTION of MYCOPLASMA SYNOVIAE isolates, as well as due to contain of RI, RII and RIII regions its can with top sensitive and operative, be used for to DIAGNOSIS and DETECTION of MYCOPLASMA SYNOVIAE isolates.

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APA: Copy

MAGHAMI, M., POURBAKHSH, S.A., HOMAYOUNIMEHR, A.R., MOHAJERANI, H.R., ASHTARI, A., & BAYATZADEH, M.A.. (2013). DIAGNOSIS AND DETECTION OF MYCOPLASMA SYNOVIAE FROM COMMERCIAL POULTRY FLOCKS USING POLYMERAS CHAIN REACTION (PCR) BASED ON AMPLIFICATION OF VLHA GENE. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 9 (NEW)(4 (39)), 837-845. SID. https://sid.ir/paper/197273/en

Vancouver: Copy

MAGHAMI M., POURBAKHSH S.A., HOMAYOUNIMEHR A.R., MOHAJERANI H.R., ASHTARI A., BAYATZADEH M.A.. DIAGNOSIS AND DETECTION OF MYCOPLASMA SYNOVIAE FROM COMMERCIAL POULTRY FLOCKS USING POLYMERAS CHAIN REACTION (PCR) BASED ON AMPLIFICATION OF VLHA GENE. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2013;9 (NEW)(4 (39)):837-845. Available from: https://sid.ir/paper/197273/en

IEEE: Copy

M. MAGHAMI, S.A. POURBAKHSH, A.R. HOMAYOUNIMEHR, H.R. MOHAJERANI, A. ASHTARI, and M.A. BAYATZADEH, “DIAGNOSIS AND DETECTION OF MYCOPLASMA SYNOVIAE FROM COMMERCIAL POULTRY FLOCKS USING POLYMERAS CHAIN REACTION (PCR) BASED ON AMPLIFICATION OF VLHA GENE,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 9 (NEW), no. 4 (39), pp. 837–845, 2013, [Online]. Available: https://sid.ir/paper/197273/en

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