DETECTION OF MYCOPLASMA SYNOVIAE IN CLINICAL SAMPLES BY VIHA-PCR METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ANSARI H.; POURBAKHSH S.A.; SHEIKHI N.; BOZORGMEHRI FARD M.H.; ASHTARI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ) Year:2010 | Volume:3 | Issue:4 (12) Page(s): 673-681

Download Full-Text

Persian Verion

View:

925

Download:

Cites:

Information Journal Paper

Title

DETECTION OF MYCOPLASMA SYNOVIAE IN CLINICAL SAMPLES BY VIHA-PCR METHOD

Author(s)

Keywords

MYCOPLASMA SYNOVIAEQ2

CLINICAL SAMPLESQ3

PCRQ2

VIHA GENEQ3

Abstract

As one of the major pathogens of avian species, MYCOPLASMA SYNOVIAE causes significant economic losses to the poultry industry. The main purpose of this study was to detect MYCOPLASMA SYNOVIAE in CLINICAL SAMPLES using the VIhA-PCR method. For serological screening test, 373 serum samples were collected from 25 breeder farms and rapid serum agglutination test conducted which revealed that 143 samples equivalent to 19 breeder farms were positive. For VIhA-PCR assay, 20 of the previously mentioned breeder farms were selected and sterile swab were collected from the palatine cleft, trachea, air sacs and lungs. Three swabs from 3 birds were placed in a test tube containing 1 ml of PBS and transferred to the laboratory for PCR test. Specific primers for VIHA GENE were employed in this study. The PCR product from specific primers showed 350-400 bp for all field isolated on electrophoresis gel in 8 farms. VIhA-PCR with high sensitivity could be employed in definitive diagnosis of MYCOPLASMA SYNOVIAE infection in the laboratory.

Cites

DIAGNOSIS AND DETECTION OF MYCOPLASMA SYNOVIAE FROM COMMERCIAL POULTRY FLOCKS USING POLYMERAS CHAIN REACTION (PCR) BASED ON AMPLIFICATION OF VLHA GENE

References

No record.

Cite

APA: Copy

ANSARI, H., POURBAKHSH, S.A., SHEIKHI, N., BOZORGMEHRI FARD, M.H., & ASHTARI, A.. (2010). DETECTION OF MYCOPLASMA SYNOVIAE IN CLINICAL SAMPLES BY VIHA-PCR METHOD. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), 3(4 (12)), 673-681. SID. https://sid.ir/paper/183091/en

Vancouver: Copy

ANSARI H., POURBAKHSH S.A., SHEIKHI N., BOZORGMEHRI FARD M.H., ASHTARI A.. DETECTION OF MYCOPLASMA SYNOVIAE IN CLINICAL SAMPLES BY VIHA-PCR METHOD. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)[Internet]. 2010;3(4 (12)):673-681. Available from: https://sid.ir/paper/183091/en

IEEE: Copy

H. ANSARI, S.A. POURBAKHSH, N. SHEIKHI, M.H. BOZORGMEHRI FARD, and A. ASHTARI, “DETECTION OF MYCOPLASMA SYNOVIAE IN CLINICAL SAMPLES BY VIHA-PCR METHOD,” VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), vol. 3, no. 4 (12), pp. 673–681, 2010, [Online]. Available: https://sid.ir/paper/183091/en

Related Journal Papers