DYNAMICS OF INTERACTION BETWEEN RAM SPERM WITH PLASMID CARRYING HUMAN LYSOZYME GENE IN SMGT

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HOSEINI PAJOOH K.; TAJIK P.; KARIMIPOUR M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Paper Information

Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2014 | Volume:11 (NEW) | Issue:3 (46) Page(s): 1331-1344

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Title

DYNAMICS OF INTERACTION BETWEEN RAM SPERM WITH PLASMID CARRYING HUMAN LYSOZYME GENE IN SMGT

Author(s)

HOSEINI PAJOOH K. | TAJIK P. | KARIMIPOUR M. | Issue Writer Certificate

Keywords

SPERMQ1

SHEEPQ1

GENE TRANSFERQ2

HUMAN LYSOZYMEQ2

Abstract

Many studies have shown SPERMatozoa can be used as vectors for transfer of exogenous DNA to oocyte to produce transgenic animals. However its efficiency is still questionable. The results of DNA concentration and incubation time on DNA uptake and SPERM functionality has been different or sometimes opposite. In this study the effect of DNA concentration and incubation time of SHEEP SPERMatozoa with foreign DNA on DNA uptake, its dynamic and SPERM functionality and their difference between ejaculated and epididimal SPERM was investigated. For this the ejaculated (N=4) and epididimal (N=6) SPERMatozoa was incubated with different concentration (5-500 ng/106 SPERM/25ml) of rhodamine labaled pEGFP-IRES-hLys in various time (15-90min) and DNA uptake, dynamic of uptake and SPERM functionality was assayed. Results showed to an average of 65% of incubated SPERMatozoa uptake the foreign DNA, depend on DNA concentration and incubation time. Although the amount of transfected SPERMatozoa could increase significantly with prolonged incubation time up to 60 min but the amount of DNA uptake was higher in the first 15min of incubation. Increase of foreign DNA concentration and incubation time increased the transfected SPERMatozoa and uptake intensity. It seems higher DNA concentration, more rapid DNA uptake and more rapid gain of transfection peak. Slight decrease of SPERM total and progressive motility was seen in treatment group in comparison with Control groups (except in 5min incubation). No SPERMatozoa with post acrosomal absorption (true uptake) were motile. So incubation for more than 30 and 60 min in moderate (20-200ng) and low (5ng) plasmid concentration respectively is not useful.

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APA: Copy

HOSEINI PAJOOH, K., TAJIK, P., & KARIMIPOUR, M.. (2014). DYNAMICS OF INTERACTION BETWEEN RAM SPERM WITH PLASMID CARRYING HUMAN LYSOZYME GENE IN SMGT. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 11 (NEW)(3 (46)), 1331-1344. SID. https://sid.ir/paper/197419/en

Vancouver: Copy

HOSEINI PAJOOH K., TAJIK P., KARIMIPOUR M.. DYNAMICS OF INTERACTION BETWEEN RAM SPERM WITH PLASMID CARRYING HUMAN LYSOZYME GENE IN SMGT. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2014;11 (NEW)(3 (46)):1331-1344. Available from: https://sid.ir/paper/197419/en

IEEE: Copy

K. HOSEINI PAJOOH, P. TAJIK, and M. KARIMIPOUR, “DYNAMICS OF INTERACTION BETWEEN RAM SPERM WITH PLASMID CARRYING HUMAN LYSOZYME GENE IN SMGT,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 11 (NEW), no. 3 (46), pp. 1331–1344, 2014, [Online]. Available: https://sid.ir/paper/197419/en

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