مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

550
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

CLONING OF HUMAN CHORIONIC GONADOTROPIN BETA SUBUNIT FOR ROOSTERSPERM MEDIATED GENE TRANSFER

Pages

  203-211

Abstract

 The aim of this study was cloning of the beta subunit of human chorionic gonadotropin in an appropriatevector for production of transgenic chicken trrough SPERM mediated gene transfer. In this regard, transgenic chicken production tecnology has taken into consideration for having many advantages such asshort generation interval, the large number of production of offspring and suitable pattern of proteinglycosylation. To date, no study has been conducted on the cloning of the beta subunit of humanchorionic gonadotropin for rooster SPERM. For this purpose, the hormone beta subunit were amplified by aspecific primer pairs, and cloned in T vector. The recombinant PLASMID was transformed into CompetentE. coli cells and colonies that containing recombinant PLASMIDs were selected by colony PCR. The validityof extracted PLASMID were analyzed by enzyme digestion and SEQUENCING. The beta chain of T vector wasisolated and was cloned again into pcDNA3. 1 + expression vector. The results of enzyme analysis andsequencing indicated that recombinant PLASMID pCDNA3. 1 +/β hCG were cloned with the correctsequence and completely matched up with human chorionic gonadotropin beta subunit gene that can beconcluded that it has sutible structure for SPERM mediated gene transfer.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    NOORANI, MAHDI, RAHIMI, SHABAN, SHAHVERDI, ABDOLHOSSEIN, & SHARAFI, MOHSEN. (2018). CLONING OF HUMAN CHORIONIC GONADOTROPIN BETA SUBUNIT FOR ROOSTERSPERM MEDIATED GENE TRANSFER. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), 20(2 ), 203-211. SID. https://sid.ir/paper/209191/en

    Vancouver: Copy

    NOORANI MAHDI, RAHIMI SHABAN, SHAHVERDI ABDOLHOSSEIN, SHARAFI MOHSEN. CLONING OF HUMAN CHORIONIC GONADOTROPIN BETA SUBUNIT FOR ROOSTERSPERM MEDIATED GENE TRANSFER. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE)[Internet]. 2018;20(2 ):203-211. Available from: https://sid.ir/paper/209191/en

    IEEE: Copy

    MAHDI NOORANI, SHABAN RAHIMI, ABDOLHOSSEIN SHAHVERDI, and MOHSEN SHARAFI, “CLONING OF HUMAN CHORIONIC GONADOTROPIN BETA SUBUNIT FOR ROOSTERSPERM MEDIATED GENE TRANSFER,” JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), vol. 20, no. 2 , pp. 203–211, 2018, [Online]. Available: https://sid.ir/paper/209191/en

    Related Journal Papers

    Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops





    Move to top