IDENTIFICATION AND DIFFERENTIATION OF VACCINAL AND VIRULENT NEWCASTLE DISEASE VIRUS IN IRAN BY HRM ANALYSIS

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DIBAZAR SH.; SHEIKHI N.; HEMMATZADEH F.; CHARKHKAR S.; POURBAKHSH A.

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Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2014 | Volume:11 (NEW) | Issue:3 (46) Page(s): 1345-1355

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Title

IDENTIFICATION AND DIFFERENTIATION OF VACCINAL AND VIRULENT NEWCASTLE DISEASE VIRUS IN IRAN BY HRM ANALYSIS

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Keywords

HRM ANALYSISQ2

NEWCASTLE DISEASE VIRUSQ2

VACCINE VIRUSQ2

DIFFERENTIATIONQ2

PRIMERQ2

Abstract

To control of outbreaks caused Newcastle disease, it is necessary to distinguish virulent viruses from vaccine strains, in minimum possible time and with high accuracy. The aim of this study was using High-Resolution Melting-Curve Analysis (HRM) for detection and DIFFERENTIATION of Iranian NEWCASTLE DISEASE VIRUS (NDVs) isolates from vaccine strains. In this study, 5 virulent isolates along with 2 vaccine strains, including B1 and Lasota were used. Based on the nucleotide sequence of F gene, 8 PRIMERs (A - H) were designed for the analysis of HRM. At the first stage, one virulent and 2 VACCINE VIRUS was analyzed by 8 PRIMER pairs. Based on the preliminary results of both RT-PCR HRM, 3 sets of the PRIMERs have been selected for final testing of the samples. The patterns obtained from wild viruses were compared with vaccine group. The melting temperatures for vaccine strains were higher than virulent isolates by A, B, C, F and H PRIMER pairs, despite D, E and G. In this study, B and H PRIMER pairs could separate vaccine strains from each other and from virulent isolates, better than other PRIMERs. Based on these results, HRM ANALYSIS and correct PRIMER selection can determinate and differentiate virulent NDVs from vaccine strains. This technique is able to do this in short time and with high accuracy in comparison with previous conventional approaches such as pathogenicity indices tests, RT-PCR and nucleotide sequencing.

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APA: Copy

DIBAZAR, SH., SHEIKHI, N., HEMMATZADEH, F., CHARKHKAR, S., & POURBAKHSH, A.. (2014). IDENTIFICATION AND DIFFERENTIATION OF VACCINAL AND VIRULENT NEWCASTLE DISEASE VIRUS IN IRAN BY HRM ANALYSIS. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 11 (NEW)(3 (46)), 1345-1355. SID. https://sid.ir/paper/197420/en

Vancouver: Copy

DIBAZAR SH., SHEIKHI N., HEMMATZADEH F., CHARKHKAR S., POURBAKHSH A.. IDENTIFICATION AND DIFFERENTIATION OF VACCINAL AND VIRULENT NEWCASTLE DISEASE VIRUS IN IRAN BY HRM ANALYSIS. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2014;11 (NEW)(3 (46)):1345-1355. Available from: https://sid.ir/paper/197420/en

IEEE: Copy

SH. DIBAZAR, N. SHEIKHI, F. HEMMATZADEH, S. CHARKHKAR, and A. POURBAKHSH, “IDENTIFICATION AND DIFFERENTIATION OF VACCINAL AND VIRULENT NEWCASTLE DISEASE VIRUS IN IRAN BY HRM ANALYSIS,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 11 (NEW), no. 3 (46), pp. 1345–1355, 2014, [Online]. Available: https://sid.ir/paper/197420/en

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