IDENTIFICATION OF NEWCASTLE DISEASE VIRUS F GENE FROM RECENT OUTBREAKS IN IRAN

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SAMADI T.; KIANI ZADEH M.; FATHI NAJAFI M.; MOUSAVI NASAB S.D.; HOSSEIN NIA DAVATGAR A.M.; JAFARI M.; AHMADI N.A.; AZIZIAN R.

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Journal: JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES Year:2013 | Volume:21 | Issue:1 Page(s): 135-142

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Title

IDENTIFICATION OF NEWCASTLE DISEASE VIRUS F GENE FROM RECENT OUTBREAKS IN IRAN

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Keywords

NEWCASTLE DISEASE VIRUSQ2

F GENE IDENTIFICATIONQ2

MUTATIONQ2

IRANQ2

Abstract

Introduction: Newcastle disease is an extremely contagious disease that infects many species of birds. NEWCASTLE DISEASE VIRUS (NDV) belongs to the paramyxoviridae family and genus Rubulavirus. The membrane of the virus particle contains HN and F glycoproteins. Cleavage of the F glycoprotein has a direct relation with the patogenicity of the virus. The aim of this study was to determine the gene and protein sequences of F glycoprotein in virus strains isolated from recent outbreaks in IRAN.Materials & Methods: Six NDV Viruses were passaged and their RNA isolated. Using RT- PCR technique, cDNA preparation and amplification of the gene were done. Isolates were assessed by electrophoresis and observed with UV. The isolates were digested with restriction enzyme Pst I. Finally, in order to corroborate the amplified PCR product, DNA of isolates were purified, dried and sequenced.Finding: In this study all of the NDV strains contained a 935 bp band that after digestion with Pst I, produce two bonds 720 bp and 180 bp. Nucleotide analysis showed single base MUTATIONs but that was not significant amino acid sequence and analysis indicated no amino acid change in the cleavage site of F protein.Discussion & Conclusions: Absence of point MUTATIONs in the cleavage site of F protein illustrated the importance of the maintained cleavage site of protein F among the strains studied.

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APA: Copy

SAMADI, T., KIANI ZADEH, M., FATHI NAJAFI, M., MOUSAVI NASAB, S.D., HOSSEIN NIA DAVATGAR, A.M., JAFARI, M., AHMADI, N.A., & AZIZIAN, R.. (2013). IDENTIFICATION OF NEWCASTLE DISEASE VIRUS F GENE FROM RECENT OUTBREAKS IN IRAN. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 21(1), 135-142. SID. https://sid.ir/paper/89846/en

Vancouver: Copy

SAMADI T., KIANI ZADEH M., FATHI NAJAFI M., MOUSAVI NASAB S.D., HOSSEIN NIA DAVATGAR A.M., JAFARI M., AHMADI N.A., AZIZIAN R.. IDENTIFICATION OF NEWCASTLE DISEASE VIRUS F GENE FROM RECENT OUTBREAKS IN IRAN. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2013;21(1):135-142. Available from: https://sid.ir/paper/89846/en

IEEE: Copy

T. SAMADI, M. KIANI ZADEH, M. FATHI NAJAFI, S.D. MOUSAVI NASAB, A.M. HOSSEIN NIA DAVATGAR, M. JAFARI, N.A. AHMADI, and R. AZIZIAN, “IDENTIFICATION OF NEWCASTLE DISEASE VIRUS F GENE FROM RECENT OUTBREAKS IN IRAN,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 21, no. 1, pp. 135–142, 2013, [Online]. Available: https://sid.ir/paper/89846/en

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