EFFECT OF RAFFINOSE AND TREHALOSE ON SPERM PARAMETERS IN BUFFALO BULLS AFTER THAWING

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PIRI BAGCHEJAKI KH.; GHIASI GHALEHKANDI J.; BEHESHTI R.

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Paper Information

Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2013 | Volume:10 (NEW) | Issue:2 (41) Page(s): 943-948

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Title

EFFECT OF RAFFINOSE AND TREHALOSE ON SPERM PARAMETERS IN BUFFALO BULLS AFTER THAWING

Author(s)

PIRI BAGCHEJAKI KH. | GHIASI GHALEHKANDI J. | BEHESHTI R. | Issue Writer Certificate

Keywords

BUFFALOQ2

RAFFINOSEQ3

SPERMATOZOAQ2

TREHALOSEQ2

Abstract

The study was designed to investigate the effect of RAFFINOSE and TREHALOSE on sperm parameters in BUFFALO bulls after thawing. For this purpose, 20 ejaculates were collected from four BUFFALO bulls in Northwest BUFFALO Research Center. Samples with excellent quality and more than 70% visual sperm motility were diluted at 37oC in Bioxcell extender with addition of (0, 25 and 75 mM) of RAFFINOSE and (0, 25 and 75 mM) TREHALOSE. The diluted semen was equilibrated at 4oC within 4 hours. Then, filled in 0.5 ml French straws and were subjected to cooling condition before being plunged into liquid nitrogen. Semen was thawed at 37oC for 40 seconds after two weeks of storage inside liquid nitrogen. Sperm motility and some quality parameters of each frozen semen sample were assessed after thawing by warm plate phase contrast microscope and by CASA evaluation. In general, the results showed that the addition of 0, 25 and 75 mM RAFFINOSE and TREHALOSE and interaction of RAFFINOSE in TREHALOSE into the Bioxcell extender for freezing BUFFALO semen did not improved the motility of SPERMATOZOA and some quality parameters after thawing (P>0.05).

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APA: Copy

PIRI BAGCHEJAKI, KH., GHIASI GHALEHKANDI, J., & BEHESHTI, R.. (2013). EFFECT OF RAFFINOSE AND TREHALOSE ON SPERM PARAMETERS IN BUFFALO BULLS AFTER THAWING. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 10 (NEW)(2 (41)), 943-948. SID. https://sid.ir/paper/197438/en

Vancouver: Copy

PIRI BAGCHEJAKI KH., GHIASI GHALEHKANDI J., BEHESHTI R.. EFFECT OF RAFFINOSE AND TREHALOSE ON SPERM PARAMETERS IN BUFFALO BULLS AFTER THAWING. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2013;10 (NEW)(2 (41)):943-948. Available from: https://sid.ir/paper/197438/en

IEEE: Copy

KH. PIRI BAGCHEJAKI, J. GHIASI GHALEHKANDI, and R. BEHESHTI, “EFFECT OF RAFFINOSE AND TREHALOSE ON SPERM PARAMETERS IN BUFFALO BULLS AFTER THAWING,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 10 (NEW), no. 2 (41), pp. 943–948, 2013, [Online]. Available: https://sid.ir/paper/197438/en

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