EFFECTS OF DIFFERENT LEVELS OF TREHALOSE AND DIFFERENT STORAGE TIMES IN ONDALAGH RAM SEMEN CHARACTERISTICS IN LIQUID CONDITION

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AKHONDI A.; MOSTAFALO Y.; MOHAMMADGHAREHBASH A.; RAHCHAMANI R.

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Journal: JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES) Year:2018 | Volume:11 | Issue:3 (42) Page(s): 1-14

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Title

EFFECTS OF DIFFERENT LEVELS OF TREHALOSE AND DIFFERENT STORAGE TIMES IN ONDALAGH RAM SEMEN CHARACTERISTICS IN LIQUID CONDITION

Author(s)

AKHONDI A. | MOSTAFALO Y. | MOHAMMADGHAREHBASH A. | RAHCHAMANI R. | Issue Writer Certificate

Keywords

TREHALOSEQ1

EXTENDERQ2

SEMENQ2

RAM DALAGHQ1

Abstract

Inroduction & Objective: Artificial insemination in sheep is an effective way to improve genetic andreproductive management. One of the vital stages of artificial insemination is the protection of ramsperm during storage. The aim of this study was to evaluate effects of different levels of TREHALOSE sugarand different storage times in Tris-chicken egg yolk and Tris-turkey egg yolk EXTENDERs on Dalagh ramsemen characteristics in liquid condition. Materials and Methods: In this study, for SEMEN sample collection four Dalagh rams in the age of 2-3 years were used. The samples were diluted in different EXTENDERs, then received different TREHALOSEsugar treatments. SEMEN samples were aspirated into 0. 5 ml straws, and were transferred into therefrigerator (5° C). Then at different times, spermatozoa characteristics including percentage of motileand viable sperm and membrane and sperm normality were evaluated. This experiment was carried outin 4×3 factorial arrangement with the use of completely randomized design. Treatments were includeddifferent levels of TREHALOSE sugar (0, 80, 100, 120 mM) and different storage times (0, 24 and 48 hours). The data were processed in Excel spread sheet environment and was analyzed by ANOVA procedure ofStatistical Analysis System SAS (version 9. 1). Comparison of data means were performed by Turkey testat the level of %1 error. Results: The results showed effects different levels of TREHALOSE sugar and different storage times inTris-chicken egg yolk and turkey egg yolk EXTENDERs on the sperm characteristics were significant(P<0. 01). Interaction of sugar levels and times on the percentage of sperm motility and membraneintegrity was significant (P<0. 01). The highest sperm motility (86. 00± 3. 45%) and viability (94. 40± 2. 74%)and sperm normality (88. 00± 2. 69%) were obtained in Tris-turkey egg yolk EXTENDER containing 120 mMTREHALOSE sugar and at 0 hours. The highest sperm membrane integrity (72. 40± 2. 66%) was obtained inTris-turkey egg yolk EXTENDER containing 0 mM TREHALOSE sugar and at 0 hours. Conclusion: For Dalagh ram SEMEN dilution, the use 120 Mm of sugar TREHALOSE in Tris-turkey eggyolk EXTENDER at the early hours of storage in liquid condition is effective.

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APA: Copy

AKHONDI, A., MOSTAFALO, Y., MOHAMMADGHAREHBASH, A., & RAHCHAMANI, R.. (2018). EFFECTS OF DIFFERENT LEVELS OF TREHALOSE AND DIFFERENT STORAGE TIMES IN ONDALAGH RAM SEMEN CHARACTERISTICS IN LIQUID CONDITION. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), 11(3 (42) ), 1-14. SID. https://sid.ir/paper/191728/en

Vancouver: Copy

AKHONDI A., MOSTAFALO Y., MOHAMMADGHAREHBASH A., RAHCHAMANI R.. EFFECTS OF DIFFERENT LEVELS OF TREHALOSE AND DIFFERENT STORAGE TIMES IN ONDALAGH RAM SEMEN CHARACTERISTICS IN LIQUID CONDITION. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES)[Internet]. 2018;11(3 (42) ):1-14. Available from: https://sid.ir/paper/191728/en

IEEE: Copy

A. AKHONDI, Y. MOSTAFALO, A. MOHAMMADGHAREHBASH, and R. RAHCHAMANI, “EFFECTS OF DIFFERENT LEVELS OF TREHALOSE AND DIFFERENT STORAGE TIMES IN ONDALAGH RAM SEMEN CHARACTERISTICS IN LIQUID CONDITION,” JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), vol. 11, no. 3 (42) , pp. 1–14, 2018, [Online]. Available: https://sid.ir/paper/191728/en

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