EVALUATION OF GENETIC DIVERSITY IN CHICKPEA LINES USING PHYSIOLOGICAL AND RAPD MOLECULAR MARKERS

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FARSHADFAR E.A.; FARSHADFAR M.

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Journal: Year:2004 | Volume:17 | Issue:2 (63 IN AGRONOMY AND HORTICULTURE) Page(s): 63-69

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Title

EVALUATION OF GENETIC DIVERSITY IN CHICKPEA LINES USING PHYSIOLOGICAL AND RAPD MOLECULAR MARKERS

Author(s)

FARSHADFAR E.A. | FARSHADFAR M. | Issue Writer Certificate

Keywords

GENETIC UNIVERSITYQ2

DROUGHT RESISTANCEQ3

RAPDQ2

STIQ4

Abstract

To investigate genetic diversity, evaluation of DROUGHT RESISTANCE and genetic polymorphism of chickpea lines in the field and molecular level using RAPD molecular marker, 21 lines of chickpea were tested in a randomized completely block design with three replications under two irrigated and rainfed conditions in the research station of Faculty of Agriculture; and research laboratory of Faculty of Science Razi University of Kermanshah. The results of analysis of variance exhibited high significant differences among the lines for yield potential (Yp), stress yield (Ys) and stress tolerance index (STI), indicating the existance of genetic variation and possibility of selection for DROUGHT RESISTANCE.Comparison of phenotypic coefficient of variation and genotypic coefficient of variation among Yp, Ys and STI showed that most of the variation was related to STI, Ys and Yp, respectively, displaying more variation in rainfed conditions. It was also observed that most of this variation was genetic. The results of mean comparison displayed that the highest potential and stress yield were belonged to the line number 11 (FLIP 92-60 C) and the most STI was related to the lines number11 and 20. Evaluation of three dimensional plot showed that the best drought resistant lines with suitable yield under rainfed and irrigated conditions were the lines 4, 7, 8, 11, 14 and 20 (group A). The results of RAPD analysis indicated the appropriateness of these techniques for determination of polymorphism among the samples. From the 5 primer used with RAPD-PCR technique, 69 polymorphic band was produced and the average band produced for each primer was 13.8 polymorphic band. According to the dendrogram resulted from the banding pattern the lines were classified into 3 groups and 8 sub-groups. According to the resulted dendrogram the farthest genetic distance was between the lines (10), and 14 with the lines 2 and 13. It is therefore suggested to get advantage of the crosses (1014), in a chickpea breeding program., 13) and (2, 14), (2,13) Comparison between the obtained dendrogram resulted from molecular experiment and the dendrogram resulted from field experiment indicated that the results of these two experiments were 71.59 in agreement with each other, providing a good background for studying drought tolerance based on the molecular markers.

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APA: Copy

FARSHADFAR, E.A., & FARSHADFAR, M.. (2004). EVALUATION OF GENETIC DIVERSITY IN CHICKPEA LINES USING PHYSIOLOGICAL AND RAPD MOLECULAR MARKERS. PAJOUHESH-VA-SAZANDEGI, 17(2 (63 IN AGRONOMY AND HORTICULTURE)), 63-69. SID. https://sid.ir/paper/20064/en

Vancouver: Copy

FARSHADFAR E.A., FARSHADFAR M.. EVALUATION OF GENETIC DIVERSITY IN CHICKPEA LINES USING PHYSIOLOGICAL AND RAPD MOLECULAR MARKERS. PAJOUHESH-VA-SAZANDEGI[Internet]. 2004;17(2 (63 IN AGRONOMY AND HORTICULTURE)):63-69. Available from: https://sid.ir/paper/20064/en

IEEE: Copy

E.A. FARSHADFAR, and M. FARSHADFAR, “EVALUATION OF GENETIC DIVERSITY IN CHICKPEA LINES USING PHYSIOLOGICAL AND RAPD MOLECULAR MARKERS,” PAJOUHESH-VA-SAZANDEGI, vol. 17, no. 2 (63 IN AGRONOMY AND HORTICULTURE), pp. 63–69, 2004, [Online]. Available: https://sid.ir/paper/20064/en

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