MOLECULAR TYPING AND PHYLOGENIC ANALYSIS OF THE LPS1-8 GENOTYPES OF PASTEURELLA MULTOCIDA ISOLATES FROM POULTRY BY LPS- PCR TYPING METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

YAZDANPOUR Z.; JABBARI A.R.; ESMAEILI ZAD M.; MESRI R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Veterinary Research & Biological Products Year:2017 | Volume:30 | Issue:2 (115) Page(s): 10-17

Download Full-Text

Persian Verion

View:

875

Download:

Cites:

Information Journal Paper

Title

MOLECULAR TYPING AND PHYLOGENIC ANALYSIS OF THE LPS1-8 GENOTYPES OF PASTEURELLA MULTOCIDA ISOLATES FROM POULTRY BY LPS- PCR TYPING METHOD

Author(s)

YAZDANPOUR Z. | JABBARI A.R. | ESMAEILI ZAD M. | MESRI R. | Issue Writer Certificate

Keywords

PASTEURELLA MULTOCIDAQ2

POULTRYQ2

GENOTYPINGQ1

LPS- PCRQ1

Abstract

PASTEURELLA MULTOCIDAis a gram-negative bacteria of veterinary importance which has divided into 16 somatic or lipopolysaccharide (LPS) serovars using an agar gel diffusion precipitation test. In this study LPS PCR typing method was used for molecular typing and phylogenic analysis of the LPS1-8 genotypes ofP. multocida isolates from POULTRY in Iran.In this study 30 isolates P. multocida were cultured on BHI medium, genomic DNA was extracted by boiling method, strains were identified by biochemical and molecular methods. LPS GENOTYPING were typed using the LPS-PCR and Specific Primers, products were sequenced and compared with the GenBank sequences. All of the isolates were typed using the LPS PCR typing.60% of isolates contained LPS1, 13.4% LPS2, 16.6% LPS3, 6/66% L1, L2, L3, 3/33% L2, L3. None of the other genotypes (L4-L8) were detected among the isolates. There were found considerable differences of nucleotide LPS genes of Iranian isolates and the related sequences in the GenBank. LPS- PCR typing showed that three LPS genotypes of L1, L2 and L3 were present among Iranian P. multocida isolates. This study showed that LPS- PCR typing was a suitable typing method for differentiating among avian P. multocida isolates based on LPS genotypes.

Cites

No record.

References

No record.

Cite

APA: Copy

YAZDANPOUR, Z., JABBARI, A.R., ESMAEILI ZAD, M., & MESRI, R.. (2017). MOLECULAR TYPING AND PHYLOGENIC ANALYSIS OF THE LPS1-8 GENOTYPES OF PASTEURELLA MULTOCIDA ISOLATES FROM POULTRY BY LPS- PCR TYPING METHOD. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 30(2 (115)), 10-17. SID. https://sid.ir/paper/200777/en

Vancouver: Copy

YAZDANPOUR Z., JABBARI A.R., ESMAEILI ZAD M., MESRI R.. MOLECULAR TYPING AND PHYLOGENIC ANALYSIS OF THE LPS1-8 GENOTYPES OF PASTEURELLA MULTOCIDA ISOLATES FROM POULTRY BY LPS- PCR TYPING METHOD. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2017;30(2 (115)):10-17. Available from: https://sid.ir/paper/200777/en

IEEE: Copy

Z. YAZDANPOUR, A.R. JABBARI, M. ESMAEILI ZAD, and R. MESRI, “MOLECULAR TYPING AND PHYLOGENIC ANALYSIS OF THE LPS1-8 GENOTYPES OF PASTEURELLA MULTOCIDA ISOLATES FROM POULTRY BY LPS- PCR TYPING METHOD,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 30, no. 2 (115), pp. 10–17, 2017, [Online]. Available: https://sid.ir/paper/200777/en

Related Journal Papers