PREPARATION OF HYPERIMMUNE SERUM AGAINST ADAPTATED PLOWRIGHT RINDERPEST VIRUS TO PRIMARY RABBIT KIDNEY CELL AND USING IN SERUM NEUTRALIZATION AND AGAR GEL IMMUNODIFFUSION TESTS

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YAGHINI M.; FALLAHI R.; KARGAR MOAKHAR R.; KHEDMATI K.

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Journal Paper

Paper Information

Journal: Year:2008 | Volume:21 | Issue:2 (79 IN ANIMAL AND FISHERIRS SCIENCES) Page(s): 17-22

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Title

PREPARATION OF HYPERIMMUNE SERUM AGAINST ADAPTATED PLOWRIGHT RINDERPEST VIRUS TO PRIMARY RABBIT KIDNEY CELL AND USING IN SERUM NEUTRALIZATION AND AGAR GEL IMMUNODIFFUSION TESTS

Author(s)

YAGHINI M. | FALLAHI R. | KARGAR MOAKHAR R. | KHEDMATI K. | Issue Writer Certificate

Keywords

HYPERIMMUNE SERUMQ3

RINDER PEST VIRUSQ3

RABBIT KIDNEY CELLQ3

AGAR GEL IMMUNODIFFUSION TESTQ3

SERUM NEUTRALIZATION TESTQ3

Abstract

Hyperimmune sera are used in diagnostic laboratories abundantly. Because of the imported antisera are expensive, the internal preparation with high quality, is very important. In this study, the HYPERIMMUNE SERUM against adaptated plowright rinderpest virus to primary RABBIT KIDNEY CELL prepared and used in AGAR GEL IMMUNODIFFUSION TEST and SERUM NEUTRALIZATION TEST. Primary cell, prepared with enzymatic disaggregation method. The attenuated virus was inoculated and the cytopathic effect was seen in 7th blind passage and it's ideal assay (10-5) obtained in 18th passage. The antiserum prepared after injection to rabbit and created clear precipitation lines in agar immunodiffusion test. In SN, the 1: 64 titer in 100 TCID50 could neutralized the RPV. The sensitivity and specificity of this antiserum was 100% and 80%, that is suitable. At the end, the adaptated virus and HYPERIMMUNE SERUM lyophilized. This serum kept to delivering to the diagnostic laboratories.

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APA: Copy

YAGHINI, M., FALLAHI, R., KARGAR MOAKHAR, R., & KHEDMATI, K.. (2008). PREPARATION OF HYPERIMMUNE SERUM AGAINST ADAPTATED PLOWRIGHT RINDERPEST VIRUS TO PRIMARY RABBIT KIDNEY CELL AND USING IN SERUM NEUTRALIZATION AND AGAR GEL IMMUNODIFFUSION TESTS. PAJOUHESH-VA-SAZANDEGI, 21(2 (79 IN ANIMAL AND FISHERIRS SCIENCES)), 17-22. SID. https://sid.ir/paper/20088/en

Vancouver: Copy

YAGHINI M., FALLAHI R., KARGAR MOAKHAR R., KHEDMATI K.. PREPARATION OF HYPERIMMUNE SERUM AGAINST ADAPTATED PLOWRIGHT RINDERPEST VIRUS TO PRIMARY RABBIT KIDNEY CELL AND USING IN SERUM NEUTRALIZATION AND AGAR GEL IMMUNODIFFUSION TESTS. PAJOUHESH-VA-SAZANDEGI[Internet]. 2008;21(2 (79 IN ANIMAL AND FISHERIRS SCIENCES)):17-22. Available from: https://sid.ir/paper/20088/en

IEEE: Copy

M. YAGHINI, R. FALLAHI, R. KARGAR MOAKHAR, and K. KHEDMATI, “PREPARATION OF HYPERIMMUNE SERUM AGAINST ADAPTATED PLOWRIGHT RINDERPEST VIRUS TO PRIMARY RABBIT KIDNEY CELL AND USING IN SERUM NEUTRALIZATION AND AGAR GEL IMMUNODIFFUSION TESTS,” PAJOUHESH-VA-SAZANDEGI, vol. 21, no. 2 (79 IN ANIMAL AND FISHERIRS SCIENCES), pp. 17–22, 2008, [Online]. Available: https://sid.ir/paper/20088/en

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