INTRASPECIFIC HYBRIDIZATIONIN POPULUS EUPHRATICA OLIV. USING IN VITRO EMBRYO RESCUE TECHNIQUE

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CALAGARI M.; JAFARI MOFIDABADI A.; TABARI M.; HOSSEINI SEYED MOHSEN

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Journal: Year:2004 | Volume:16 | Issue:4 (61 IN NATURAL RESOURCES) Page(s): 6-9

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Information Journal Paper

Title

INTRASPECIFIC HYBRIDIZATIONIN POPULUS EUPHRATICA OLIV. USING IN VITRO EMBRYO RESCUE TECHNIQUE

Author(s)

CALAGARI M. | JAFARI MOFIDABADI A. | TABARI M. | HOSSEINI SEYED MOHSEN | Issue Writer Certificate

Keywords

POPULUS EUPHRATICAQ4

INTRASPECIFIC HYBRIDIZATIONQ4

EMBRYO RESCUEQ4

GEOGRAPHICAL VARIATIONQ3

OVARY AND OVULE GERMINATIONQ4

Abstract

In order to investigate the effect of different geographical origin of pollen grain in INTRASPECIFIC HYBRIDIZATION and to achieve hybrids vigor, artificial hybridization has been carried out between different local genotype of POPULUS EUPHRATICA Oliv. from some wild growing stands of Iran. Because of long period required for embryo development on maternal plant, 45 days old ovary and ovule were used for in vitro culture. The isolated ovary and ovule were then transferred to the different kind of media (MS, Half MS and DKW). Significant difference (p=0.05) were observed between efficiency of pollinated ovaries (34.03%) and ovalses (47.47%) to produce plantlets. In spite of no significant difference between media used for embryo germination, the highest percentage of embryo germination was observed in MS medium (43.22%). Due to exist several numbers of ovales in each ovary (15-30), the highest number of plantlets was observed in ovary culture. No significant differences were observed between different origin of pollen grain (geographical regions) on percentage of embryo fertilization and germination. One hundred thirty seven plantlets were successfully acclimatized in a green house and transferred to the Field.

Cites

EMBRYO CULTURE AS A TOOL IN INTERGENERIC HYBRIDIZATION OF SALICACEAE (SALIX ALBA X POPULUS CASPICA)

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APA: Copy

CALAGARI, M., JAFARI MOFIDABADI, A., TABARI, M., & HOSSEINI, SEYED MOHSEN. (2004). INTRASPECIFIC HYBRIDIZATIONIN POPULUS EUPHRATICA OLIV. USING IN VITRO EMBRYO RESCUE TECHNIQUE. PAJOUHESH-VA-SAZANDEGI, 16(4 (61 IN NATURAL RESOURCES)), 6-9. SID. https://sid.ir/paper/20110/en

Vancouver: Copy

CALAGARI M., JAFARI MOFIDABADI A., TABARI M., HOSSEINI SEYED MOHSEN. INTRASPECIFIC HYBRIDIZATIONIN POPULUS EUPHRATICA OLIV. USING IN VITRO EMBRYO RESCUE TECHNIQUE. PAJOUHESH-VA-SAZANDEGI[Internet]. 2004;16(4 (61 IN NATURAL RESOURCES)):6-9. Available from: https://sid.ir/paper/20110/en

IEEE: Copy

M. CALAGARI, A. JAFARI MOFIDABADI, M. TABARI, and SEYED MOHSEN HOSSEINI, “INTRASPECIFIC HYBRIDIZATIONIN POPULUS EUPHRATICA OLIV. USING IN VITRO EMBRYO RESCUE TECHNIQUE,” PAJOUHESH-VA-SAZANDEGI, vol. 16, no. 4 (61 IN NATURAL RESOURCES), pp. 6–9, 2004, [Online]. Available: https://sid.ir/paper/20110/en

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