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Information Journal Paper

Title

DETERMINATION OF THE MOST SUITABLE CULTURE MEDIUM AND GROWTH CONDITIONS FOR MICROPROPAGATION OF GF677 (HYBRID OF ALMOND X PEACH) ROOTSTOCKS

Pages

  234-243

Keywords

ALMOND PEACH HYBRID (GF677)Q2

Abstract

 In the present investigation in vitro propagation of GF677 (Hybrid of almond x peach) rootstock was studied. Explants were isolated from apical and lateral buds early April. Plant materials were then sterilized using Hg Cl2 (0.1%) for 6 minutes. The culture media MS, 1/2 MS and modified Knop (with some changes in microelements) were used in experiments. The best shoot proliferation achieved on modified Knop medium containing 2% sucrose, 1mgll BA, without NAA (0.0). In this medium about 5 shoots per test tube were produced and there were significant difference between hormone concentrates at 0 1% level. The conditions of growth chamber was 16-h light period and a 24/26oC day/night temperature regime. Light intensity was 2500-3000 lux and air humidity was 45%. In rooting stage, use of LS medium containing 0.3 mg/l IBA , 1.6 mg/l thiamin and 7 days darkness period, gave the best results of rooting, up to 80% . Propagated plants via tissue culture were transferred to the soil consisting of 40% peat and 60 % sand mixture, or Jiffy blocks, but Jiffy blocks gave better results.

Cites

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  • Cite

    APA: Copy

    KAMALI, K., MAHIDI, E., & ZARGHAMI, R.. (2001). DETERMINATION OF THE MOST SUITABLE CULTURE MEDIUM AND GROWTH CONDITIONS FOR MICROPROPAGATION OF GF677 (HYBRID OF ALMOND X PEACH) ROOTSTOCKS. SEED AND PLANT, 17(3), 234-243. SID. https://sid.ir/paper/20313/en

    Vancouver: Copy

    KAMALI K., MAHIDI E., ZARGHAMI R.. DETERMINATION OF THE MOST SUITABLE CULTURE MEDIUM AND GROWTH CONDITIONS FOR MICROPROPAGATION OF GF677 (HYBRID OF ALMOND X PEACH) ROOTSTOCKS. SEED AND PLANT[Internet]. 2001;17(3):234-243. Available from: https://sid.ir/paper/20313/en

    IEEE: Copy

    K. KAMALI, E. MAHIDI, and R. ZARGHAMI, “DETERMINATION OF THE MOST SUITABLE CULTURE MEDIUM AND GROWTH CONDITIONS FOR MICROPROPAGATION OF GF677 (HYBRID OF ALMOND X PEACH) ROOTSTOCKS,” SEED AND PLANT, vol. 17, no. 3, pp. 234–243, 2001, [Online]. Available: https://sid.ir/paper/20313/en

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