STERILIZATION, ESTABLISHMENT AND PROLIFERATION OF SOME PRUNUS INTERSPESIFIC HYBRIDS FOR IN VITRO CULTURE

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DEZHAMPOUR JALIL; GERIGOURIAN V.; MAJIDI HERAVAN E.; ALI ASGHARZADEH N.

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Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2007 | Volume:8 | Issue:3 Page(s): 165-174

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Title

STERILIZATION, ESTABLISHMENT AND PROLIFERATION OF SOME PRUNUS INTERSPESIFIC HYBRIDS FOR IN VITRO CULTURE

Author(s)

DEZHAMPOUR JALIL | GERIGOURIAN V. | MAJIDI HERAVAN E. | ALI ASGHARZADEH N. | Issue Writer Certificate

Keywords

IN VITRO CULTUREQ2

INTERSPESIFIC HYBRIDSQ3

PRUNUSQ3

Abstract

In this research, establishment and determination of optimal medium with suitable hormone balance for proliferation of 4 INTERSPESIFIC HYBRIDS were studied in the IN VITRO CULTURE. The explants were taken from lateral buds and shoot tips of HS314 (P. amygdalus × P. persica), HS 302 (P. armeniaca × P. cerasifera), HS408 (P. armeniaca × P. domestica), HS721 (P. amygdalus × P. cerasifera) and GF677 (as control) during growth and dormant seasons. Single node cuttings were used as explant and sterilized by sodium hypochlorite solution (CloNa) and mercuric chloride (HgCl2) then cultured in four culture media: QL, modified Knop, WPM and modified MS. The results showed that sodium hypochlorite (2.5%) in 5, 7 and 10 min and HgCl2 with 0.5, 0.8 and 1 g l-1 (in 5 min) were the best treatment for herbaceous, semihard wood and hardwood explants, respectively. In establishment stage WPM for HS302 and HS408 and modified MS for HS314, HS721 and GF677 with 0.5 mg l-1 BAP + 0.05 mg l-1 NAA were the optimal culture media. In proliferation stage, modified MS and half-strength MS with 1.5 mg l-1 BAP for HS314, HS721 and GF677 and WPM with 1 mg l-1 + 0.01 mg l-1 IBA for HS302 and HS408 were the best culture media and concentrations of growth regulators. Therefore, HS314 and HS302 had high potential for growth rate and proliferation while HS408 and HS721 were low proliferation potential.

Cites

EVALUATION OF IN VITRO ROOTING OF SOME INTERSPECIFIC HYBRIDS IN PRUNUS GENUS

References

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APA: Copy

DEZHAMPOUR, JALIL, GERIGOURIAN, V., MAJIDI HERAVAN, E., & ALI ASGHARZADEH, N.. (2007). STERILIZATION, ESTABLISHMENT AND PROLIFERATION OF SOME PRUNUS INTERSPESIFIC HYBRIDS FOR IN VITRO CULTURE. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 8(3), 165-174. SID. https://sid.ir/paper/80677/en

Vancouver: Copy

DEZHAMPOUR JALIL, GERIGOURIAN V., MAJIDI HERAVAN E., ALI ASGHARZADEH N.. STERILIZATION, ESTABLISHMENT AND PROLIFERATION OF SOME PRUNUS INTERSPESIFIC HYBRIDS FOR IN VITRO CULTURE. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2007;8(3):165-174. Available from: https://sid.ir/paper/80677/en

IEEE: Copy

JALIL DEZHAMPOUR, V. GERIGOURIAN, E. MAJIDI HERAVAN, and N. ALI ASGHARZADEH, “STERILIZATION, ESTABLISHMENT AND PROLIFERATION OF SOME PRUNUS INTERSPESIFIC HYBRIDS FOR IN VITRO CULTURE,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 8, no. 3, pp. 165–174, 2007, [Online]. Available: https://sid.ir/paper/80677/en

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