THE EFFECT OF GINGER COMPARED WITH VITAMIN E ON IN VITRO CULTURE OF SPERMATOGONIAL STEM CELLS (SSCS) OF AFSHARI SHEEP

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BEYRANVAND ELHAM; SANJABI MOHAMMAD REZA; ZANDI MOHAMMAD; OFOGHI HAMIDEH

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Journal: Journal of Animal Production Year:2017 | Volume:19 | Issue:2 Page(s): 493-506

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Title

THE EFFECT OF GINGER COMPARED WITH VITAMIN E ON IN VITRO CULTURE OF SPERMATOGONIAL STEM CELLS (SSCS) OF AFSHARI SHEEP

Author(s)

BEYRANVAND ELHAM | SANJABI MOHAMMAD REZA | ZANDI MOHAMMAD | OFOGHI HAMIDEH | Issue Writer Certificate

Keywords

ANTIOXIDANTQ2

APOPTOSISQ1

GINGERQ1

SPERMATOGONIAL STEM CELLSQ1

VITAMIN EQ2

Abstract

The goal of this paper was to study the effect of GINGER extracts and VITAMIN E on the performance of ovine SPERMATOGONIAL STEM CELLS (SSCs). For this purpose, different concentrations of hydro and hydroethanolic extracts of GINGER and VITAMIN E on viability, colony formation and expression of inhibiting (bcl2ll and bcl2) and inducing (bax) APOPTOSIS genes were studied. SPERMATOGONIAL STEM CELLS (SSCs) were extracted from lamb testes with slaughterhouse origin using two steps enzymatic digestion method and enrichment by differential plating method. The characterization of SSCs was carried by alkaline phosphatase staining and expression of c-kit and oct-4 genes. Results have shown that the viability of SSCs was decreased significantly by using more than 800 μ g/mL of hydroethanolic extract of GINGER, in comparision with control group (p<0. 05), while 800 μ g/mL of hydro extract of GINGER has been resulted to be the most viable cells, and increasing the VITAMIN E concentration (upto 100 μ g/mL) resulted to be the most survival of SSCs. Expression of bax in effective concentration of hydro extract of GINGER (150 μ g/mL) and VITAMIN E (50 μ g/mL) was decreased, but hydroethanolic extract of GINGER (150 μ g/mL) and higher concentration of hydro extract of GINGER (more than 800 μ g/mL) increased the expression od bax. Based on the results of current study, 150 μ g/mL of aqueous extract of GINGER or 50 μ g/mL of VITAMIN E can be used to improve ovine SSC culture system.

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APA: Copy

BEYRANVAND, ELHAM, SANJABI, MOHAMMAD REZA, ZANDI, MOHAMMAD, & OFOGHI, HAMIDEH. (2017). THE EFFECT OF GINGER COMPARED WITH VITAMIN E ON IN VITRO CULTURE OF SPERMATOGONIAL STEM CELLS (SSCS) OF AFSHARI SHEEP. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), 19(2 ), 493-506. SID. https://sid.ir/paper/209138/en

Vancouver: Copy

BEYRANVAND ELHAM, SANJABI MOHAMMAD REZA, ZANDI MOHAMMAD, OFOGHI HAMIDEH. THE EFFECT OF GINGER COMPARED WITH VITAMIN E ON IN VITRO CULTURE OF SPERMATOGONIAL STEM CELLS (SSCS) OF AFSHARI SHEEP. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE)[Internet]. 2017;19(2 ):493-506. Available from: https://sid.ir/paper/209138/en

IEEE: Copy

ELHAM BEYRANVAND, MOHAMMAD REZA SANJABI, MOHAMMAD ZANDI, and HAMIDEH OFOGHI, “THE EFFECT OF GINGER COMPARED WITH VITAMIN E ON IN VITRO CULTURE OF SPERMATOGONIAL STEM CELLS (SSCS) OF AFSHARI SHEEP,” JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), vol. 19, no. 2 , pp. 493–506, 2017, [Online]. Available: https://sid.ir/paper/209138/en

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