DETECTION OF POLYMORPHISM DOPAMINE D1 RECEPTOR GENE IN KHOUZESTAN NATIVE CHICKENS USING PCR-RFLP

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BAZGIRI MARYAM; BEIGI NASSIRI MOHAMMAD TAGHI; FAYAZI JAMAL

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Journal: Journal of Animal Production Year:2017 | Volume:19 | Issue:1 Page(s): 13-22

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Title

DETECTION OF POLYMORPHISM DOPAMINE D1 RECEPTOR GENE IN KHOUZESTAN NATIVE CHICKENS USING PCR-RFLP

Author(s)

BAZGIRI MARYAM | BEIGI NASSIRI MOHAMMAD TAGHI | FAYAZI JAMAL | Issue Writer Certificate

Keywords

BROODINESSQ2

GENEDOPAMINEQ1

NATIVE HENSQ1

POLYMORPHISMQ1

Abstract

This study was conducted to determine the POLYMORPHISM of dopamine D1 receptor gene POLYMORPHISM using PCR-RFLP method in Khouzestan native chicken. In this study, blood samples were collected randomly from 100 native Khouzestani hens and genomic DNA was extracted using modified salting out method. Polymerase chain reaction was used to amplify 283 bp fragments of dopamine D1 receptor gene promoter by specific primers. The amplified fragment was digested with CfrI enzyme. Allele frequencies for G and A were 0. 39 and 0. 61, and genotype frequencies for AA, GG and AG were 0. 40, 0. 18 and 0. 42, respectively. Chi-square (X2) analysis indicated that genotype frequency of this locus is not in Hardy-Weinberg equilibrium. The sequencing results showed two mutations of type A to G in the base 123 and C to T in the base 198. The results of sequencing confirmed that the restriction enzyme CfrI has ability to identify the correct mutations.

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APA: Copy

BAZGIRI, MARYAM, BEIGI NASSIRI, MOHAMMAD TAGHI, & FAYAZI, JAMAL. (2017). DETECTION OF POLYMORPHISM DOPAMINE D1 RECEPTOR GENE IN KHOUZESTAN NATIVE CHICKENS USING PCR-RFLP. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), 19(1 ), 13-22. SID. https://sid.ir/paper/209169/en

Vancouver: Copy

BAZGIRI MARYAM, BEIGI NASSIRI MOHAMMAD TAGHI, FAYAZI JAMAL. DETECTION OF POLYMORPHISM DOPAMINE D1 RECEPTOR GENE IN KHOUZESTAN NATIVE CHICKENS USING PCR-RFLP. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE)[Internet]. 2017;19(1 ):13-22. Available from: https://sid.ir/paper/209169/en

IEEE: Copy

MARYAM BAZGIRI, MOHAMMAD TAGHI BEIGI NASSIRI, and JAMAL FAYAZI, “DETECTION OF POLYMORPHISM DOPAMINE D1 RECEPTOR GENE IN KHOUZESTAN NATIVE CHICKENS USING PCR-RFLP,” JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), vol. 19, no. 1 , pp. 13–22, 2017, [Online]. Available: https://sid.ir/paper/209169/en

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