Genotypic Diversity Of Dopamine D1 Receptor Gene (DRD1) In Indigenous Chickens Of Marandi And Mazandarani Breeds

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Pish Jang Aghajeri j.; Badakhshan z.; Tirandaz s.

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Journal: Veterinary Research & Biological Products Year:2019 | Volume:32 | Issue:2 (123) Page(s): 19-25

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Title

Genotypic Diversity Of Dopamine D1 Receptor Gene (DRD1) In Indigenous Chickens Of Marandi And Mazandarani Breeds

Author(s)

Pish Jang Aghajeri j. | Badakhshan z. | Tirandaz s. | Issue Writer Certificate

Keywords

PolymorphismQ2

DRD1 geneQ1

BroodinessQ1

Indigenous chickenQ2

Abstract

Prolactin gene plays a key role in the initiation and continuation of Broodiness in chickens. Dopamine in the pituitary gland reduces the effect of prolactin. This research was conducted to investigate the allelic Polymorphism of dopamine D1 receptor gene (DRD1) using PCR-RFLP method in Marandi and Mazandarani Indigenous chickens. In this study, blood samples were collected randomly from 200 chickens and genomic DNA was extracted using salting out method. Amplification of the desired locus with 283 bp was performed using specific primers and the CfrI enzyme was used to identify the mutation in the desired locus. After digestion, for DRD1 marker site, three genotypes AA, AG and GG, and two alleles of A (with on band of 283 bp) and G (with two bands of 183 and 100 bp) were identified. Indigenous chicken masses were in the Hardy-Weinberg equilibrium. For Marandi and Mazandarani Indigenous chicken masses, the Shannon information index in DRD1 marker site was 0. 59 and 0. 67, respectively, the fixation index was-0. 09 and-0. 19, respectively and the observed heterozygosity index was 0. 44 and 0. 58, respectively. Regarding the presence of Polymorphism and mutation in the studied locus and by studying the traits of related to the observed genotypes, it is suggested that this locus can be used as an appropriate marker for breeding programs.

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APA: Copy

Pish Jang Aghajeri, j., Badakhshan, z., & Tirandaz, s.. (2019). Genotypic Diversity Of Dopamine D1 Receptor Gene (DRD1) In Indigenous Chickens Of Marandi And Mazandarani Breeds. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 32(2 (123) ), 19-25. SID. https://sid.ir/paper/200774/en

Vancouver: Copy

Pish Jang Aghajeri j., Badakhshan z., Tirandaz s.. Genotypic Diversity Of Dopamine D1 Receptor Gene (DRD1) In Indigenous Chickens Of Marandi And Mazandarani Breeds. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2019;32(2 (123) ):19-25. Available from: https://sid.ir/paper/200774/en

IEEE: Copy

j. Pish Jang Aghajeri, z. Badakhshan, and s. Tirandaz, “Genotypic Diversity Of Dopamine D1 Receptor Gene (DRD1) In Indigenous Chickens Of Marandi And Mazandarani Breeds,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 32, no. 2 (123) , pp. 19–25, 2019, [Online]. Available: https://sid.ir/paper/200774/en

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