INVESTIGATION OF WNT10A AND WNT10B GENES POLYMORPHISM IN AFSHARI ×BOOROOLA MERINO CROSSBRED SHEEP

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ZAMANI AFSHAR SOMAYEH; HARKINEZHAD TAHER; BAHARI ABBAS; SHAHIR MOHAMAD HOSSEIN

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Journal: Journal of Animal Production Year:2018 | Volume:20 | Issue:2 Page(s): 213-223

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Title

INVESTIGATION OF WNT10A AND WNT10B GENES POLYMORPHISM IN AFSHARI ×BOOROOLA MERINO CROSSBRED SHEEP

Author(s)

ZAMANI AFSHAR SOMAYEH | HARKINEZHAD TAHER | BAHARI ABBAS | SHAHIR MOHAMAD HOSSEIN | Issue Writer Certificate

Keywords

AFSHARI SHEEPQ1

CARCASS TRAITSQ2

POLYMORPHISMQ1

SNPQ1

WNT GENEQ2

Abstract

Selection of live animals with minimum carcass fat in animal breeding programs will lead to increase inmeat production at national level. The ligands of WNT GENEs are of the effective factors in adipocyte celldifferentiation. This study was aimed to assess the association between POLYMORPHISMs in Wnt10a andWnt10b genes and CARCASS TRAITS in 96 Afshari – Booroola Merino male lambs. In this study, DNA wasextracted from blood samples using phenol-chloroform extraction method and polymerase chain reactionwas performed for amplification a 663 bp fragment of exon III and a part of the second intron of Wnt10agene and a 512 bp fragment of exon III Wnt10b gene. The results showed a POLYMORPHISM and threemonomorphisms (a different allele compared to reference sequence of the gene) in the Wnt10a gene butall the sequences of the Wnt10b fragment were the same in studied region of the gene. Results of thesequencing lead to identification of four single nucleotide changes in Wnt10a gene in the studied areawhen compared to the reference sequence. One of the monomorphisms was in the intron and among otherthree nucleotide changes identified in exon III one was a missense in codon 139. After digestion withrestriction enzymes HpaII at this codon, it was observed that all sampled lambs had mutant homozygousgenotype compared to the reference sequence. This site was also evaluated in a number of Afshari (fattailed)and Zell (with the least fat tail) but again the same results were observed. Therefore, it seems thatin this position the G is the wild type allele in the studied population.

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APA: Copy

ZAMANI AFSHAR, SOMAYEH, HARKINEZHAD, TAHER, BAHARI, ABBAS, & SHAHIR, MOHAMAD HOSSEIN. (2018). INVESTIGATION OF WNT10A AND WNT10B GENES POLYMORPHISM IN AFSHARI ×BOOROOLA MERINO CROSSBRED SHEEP. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), 20(2 ), 213-223. SID. https://sid.ir/paper/209180/en

Vancouver: Copy

ZAMANI AFSHAR SOMAYEH, HARKINEZHAD TAHER, BAHARI ABBAS, SHAHIR MOHAMAD HOSSEIN. INVESTIGATION OF WNT10A AND WNT10B GENES POLYMORPHISM IN AFSHARI ×BOOROOLA MERINO CROSSBRED SHEEP. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE)[Internet]. 2018;20(2 ):213-223. Available from: https://sid.ir/paper/209180/en

IEEE: Copy

SOMAYEH ZAMANI AFSHAR, TAHER HARKINEZHAD, ABBAS BAHARI, and MOHAMAD HOSSEIN SHAHIR, “INVESTIGATION OF WNT10A AND WNT10B GENES POLYMORPHISM IN AFSHARI ×BOOROOLA MERINO CROSSBRED SHEEP,” JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), vol. 20, no. 2 , pp. 213–223, 2018, [Online]. Available: https://sid.ir/paper/209180/en

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