EFFECT OF CO-INOCULATION OF SINORHIZOBIUM SP. AND PLANT GROWTH-PROMOTING RHIZOBACTERIA ON N-FIXATION AND GROWTH OF ALFALFA

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EBRAHIMI M.; AKHGAR A.R.

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Journal: Journal of Soil Management and Sustainable Production Year:2014 | Volume:4 | Issue:1 Page(s): 183-199

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Title

EFFECT OF CO-INOCULATION OF SINORHIZOBIUM SP. AND PLANT GROWTH-PROMOTING RHIZOBACTERIA ON N-FIXATION AND GROWTH OF ALFALFA

Author(s)

EBRAHIMI M. | AKHGAR A.R. | Issue Writer Certificate

Keywords

NITROGEN FIXATIONQ2

SINORHIZOBIUM SP.Q2

PGPRQ2

ALFALFAQ2

Abstract

Symbiotic biological NITROGEN FIXATION (BNF) is beneficial for increasing yield of legums, reduction of production expenses, increment of soil fertility, and prevention of underground water pollution. In the first stage of this study, the symbiotic effectiveness (S.E.) of 31Sinorhizobium isolates with ALFALFA plant was evaluated. The results indicated that 27% was very efficient, 23% efficient, 16% relatively efficient and 36% non efficient. The SR42 and SR40 were the most and least effective isolates on ALFALFA, respectively. Isolates SR8, SR14, SR15, SR26, SR51, SR52 and SR75 were effective on ALFALFA plant as well. According to the results obtained from evaluation of effective indices in symbiosis, SR42 isolate was chosen and then in a greenhouse experiment the effect of co-inoculation Sinorhizobium isolate (SR42) and two plant growth-promoting rhizobacteria including P. fluorescens P9 and P. fluorescens P52 (isolated from canola and pistachio rhizosphere, respectively) on nodulation, NITROGEN FIXATION, plant biomass weight and uptake of some elements was investigated. The results showed that application of PGPRs significantly increased shoot dry weight, nodulation number and total N uptake in ALFALFA inoculated with SINORHIZOBIUM SP. SR42.

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