MOLECULAR STUDY OF CALPASTAIN, CALPAIN AND BETA-LACTO GLOBULIN LOCI IN KORDI SHEEP

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NASSIRY M.R.; VALIZADEH R.; TAHMOORESPUR M.; JAVADMANESH A.; FOROUTANI S.

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Journal: Iranian Journal of Animal Science Research Year:2010 | Volume:2 | Issue:2 Page(s): 163-170

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Title

MOLECULAR STUDY OF CALPASTAIN, CALPAIN AND BETA-LACTO GLOBULIN LOCI IN KORDI SHEEP

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Keywords

BETA-LACTO GLOBULINQ2

CALPASTATINQ3

CALPAINQ3

POLYMORPHISMQ2

KORDI SHEEPQ2

Abstract

The genotypes for BETA-LACTO GLOBULIN (Blg) and CALPASTATIN (Cast) were determined by polymerase chain reaction (PCR) and restriction enzyme digestion and genotyped for CALPAIN (Capn) by PCR-SSCP method in a native Iranian breed sheep, Kordi. Blood samples were collected from 100 pure bred KORDI SHEEP from Kordi breeding station located in Shirvan, Mashhad. The extraction of genomic DNA was based on Guanidine Thiocyanate-Silica gel method. After PCR reaction, amplicons were digested with restriction enzymes MspI and RsaI for BETA-LACTO GLOBULIN and CALPASTATIN genes, respectively. The betalactoglobulin locus had 3 genotypes with frequencies of 0.24, 0.54 and 0.22 for AA, AB and BB, respectively, CALPASTATIN genotypes had 2 genotypes with frequencies of 0.76 and 0.24 for MM and MN genotypes, respectively. CALPAIN genotypes were analyzed with SSCP method, which was 2 genotypes with frequencies of 0.92 and 0.08 for AA and AB, respectively. Heterozygosity value for BETA-LACTO GLOBULIN locus was 50% and for CALPASTATIN and CALPAIN loci was very low (21% and 8% respectively). c2 test confirmed the Hardy-Weinberg equilibrium for three loci in this population. These data provide evidence that Iranian's KORDI SHEEP breed have a variability, which opens interesting prospects for future selection programs, especially marker-assistant selection between different genotypes of different locus and milk and chess characteristics, gain and meat traits and also for preservation strategies.

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APA: Copy

NASSIRY, M.R., VALIZADEH, R., TAHMOORESPUR, M., JAVADMANESH, A., & FOROUTANI, S.. (2010). MOLECULAR STUDY OF CALPASTAIN, CALPAIN AND BETA-LACTO GLOBULIN LOCI IN KORDI SHEEP. IRANIAN JOURNAL OF ANIMAL SCIENCE RESEARCH, 2(2), 163-170. SID. https://sid.ir/paper/218334/en

Vancouver: Copy

NASSIRY M.R., VALIZADEH R., TAHMOORESPUR M., JAVADMANESH A., FOROUTANI S.. MOLECULAR STUDY OF CALPASTAIN, CALPAIN AND BETA-LACTO GLOBULIN LOCI IN KORDI SHEEP. IRANIAN JOURNAL OF ANIMAL SCIENCE RESEARCH[Internet]. 2010;2(2):163-170. Available from: https://sid.ir/paper/218334/en

IEEE: Copy

M.R. NASSIRY, R. VALIZADEH, M. TAHMOORESPUR, A. JAVADMANESH, and S. FOROUTANI, “MOLECULAR STUDY OF CALPASTAIN, CALPAIN AND BETA-LACTO GLOBULIN LOCI IN KORDI SHEEP,” IRANIAN JOURNAL OF ANIMAL SCIENCE RESEARCH, vol. 2, no. 2, pp. 163–170, 2010, [Online]. Available: https://sid.ir/paper/218334/en

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