HORIZONTAL POLYACRYLAMIDE GEL ELECTROPHORESIS BY AGAROSE SLOTS

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SAFARI MOHAMMAD HOSSEIN; MIRZAIE ASL ASGHAR

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Journal: Agricultural Biotechnology Year:2015 | Volume:14 | Issue:1 Page(s): 33-37

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Information Journal Paper

Title

HORIZONTAL POLYACRYLAMIDE GEL ELECTROPHORESIS BY AGAROSE SLOTS

Author(s)

SAFARI MOHAMMAD HOSSEIN | MIRZAIE ASL ASGHAR | Issue Writer Certificate

Keywords

HORIZONTAL ELECTROPHORESISQ2

POLYACRYLAMIDE GELQ2

AGAROSE GELQ1

Abstract

Horizontal submerged AGAROSE GEL electrophoresis of nucleic acids is a basic tool of modern molecular biology. This method is quick and easy and the use of that does not require too much skills. Separation of small fragments of DNA and molecular proteins are not possible with AGAROSE GEL efficiently, so that acrylamide gel is used for the above mentioned aims. Vertical POLYACRYLAMIDE GEL electrophoresis, which is costly, time consuming with hard handling. Recently, a method of horizontal POLYACRYLAMIDE GEL electrophoresis was introduced which requires especial equipment. In this study, we used the agarose electrophoresis tank with changes for small DNA fragments of SSR markers separation by horizontal POLYACRYLAMIDE GEL electrophoresis. In this method, HORIZONTAL ELECTROPHORESIS performed using by a two-part gel prepared with agarose and polyacrylamide. The results showed the very sharp bands and in addition, in this electrophoresis method air bubbles not formed and cause reduction rupturing in the gel by glasses separation. New devised electrophoresis system can be applied for two aims, agarose and POLYACRYLAMIDE GELs electrophoresis.

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APA: Copy

SAFARI, MOHAMMAD HOSSEIN, & MIRZAIE ASL, ASGHAR. (2015). HORIZONTAL POLYACRYLAMIDE GEL ELECTROPHORESIS BY AGAROSE SLOTS. AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH), 14(1), 33-37. SID. https://sid.ir/paper/219871/en

Vancouver: Copy

SAFARI MOHAMMAD HOSSEIN, MIRZAIE ASL ASGHAR. HORIZONTAL POLYACRYLAMIDE GEL ELECTROPHORESIS BY AGAROSE SLOTS. AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH)[Internet]. 2015;14(1):33-37. Available from: https://sid.ir/paper/219871/en

IEEE: Copy

MOHAMMAD HOSSEIN SAFARI, and ASGHAR MIRZAIE ASL, “HORIZONTAL POLYACRYLAMIDE GEL ELECTROPHORESIS BY AGAROSE SLOTS,” AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH), vol. 14, no. 1, pp. 33–37, 2015, [Online]. Available: https://sid.ir/paper/219871/en

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