EVALUATION AND COMPARISON OF HYDATID CYST FLUID AND PROTOSCOLEX ANTIGENS TO DIFFERENT INTERMEDIATE HOSTS AND HUMAN BY SDS-PAGE GEL ELECTROPHORESIS AND WESTERN BLOTTING

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RAFIEI A.; DEZFULINEJAD M.; MIRZAEI ASAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Journal of Ilam University of Medical Sciences Year:2005 | Volume:13 | Issue:1 Page(s): 3-12

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Title

EVALUATION AND COMPARISON OF HYDATID CYST FLUID AND PROTOSCOLEX ANTIGENS TO DIFFERENT INTERMEDIATE HOSTS AND HUMAN BY SDS-PAGE GEL ELECTROPHORESIS AND WESTERN BLOTTING

Author(s)

RAFIEI A. | DEZFULINEJAD M. | MIRZAEI ASAD | Issue Writer Certificate

Keywords

ECHINOCOCCUS GRANULOSUSQ3

HYDATID CYSTQ3

PROTOSCOLEXQ3

Abstract

Introduction: Echinococus granulosus is a causdtive agent of cystic echinococcosis (CE) in some herbivorous animals and human beings. Different life cycle strains or poesies have been indicated by gentic evaluation all over the world. However, littile information is available on the antigenic differences between these strains. The aim of this study was to assess and compare the PROTOSCOLEX to HYDATID CYST fluid antiges of sheep, buffalo, bovin, camel and human by (SDS-PA GE) electrophoresis and Immunobloting. In addition, the presence of antibodies against different evaluated antigens was investigated using confirmed CE sera in western blotting.Methods: In the present study, 131 HYDATID CYST fluid and 45 PROTOSCOLEX samples of sheep, bovin, buffalo, camel and human were evaluated. HYDATID CYST fluid and PROTOSCOLEX from the above mentioned samples indicated bands with the ranges of 8 to 162 kDa.Results: The results indicated some differences between cyst fluid samples taken from different animal sources or even within samples from the same animals by electrophoresis. Immunobloting techniques confirmed differences between different animal sources, but there were no significant differences among animals from the same species. There was no significant difference in protoscoleces samples from all sources by electrophoresis, but Immunobloting have shown different antigenic bands in camel samples.Conclusion. Existence of similar PROTOSCOLEX patern in sheep, bovin, buffalo and human sources may be result of sheep-dog strain infection. Meanwhile, existence of some extra band in camel sources in both HYDATID CYST fluid and PROTOSCOLEX and absence of these bands in other examined sources from different mammals and human begins, could confirm possibility of antigenic variations in camel strain.

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APA: Copy

RAFIEI, A., DEZFULINEJAD, M., & MIRZAEI, ASAD. (2005). EVALUATION AND COMPARISON OF HYDATID CYST FLUID AND PROTOSCOLEX ANTIGENS TO DIFFERENT INTERMEDIATE HOSTS AND HUMAN BY SDS-PAGE GEL ELECTROPHORESIS AND WESTERN BLOTTING. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 13(1), 3-12. SID. https://sid.ir/paper/90238/en

Vancouver: Copy

RAFIEI A., DEZFULINEJAD M., MIRZAEI ASAD. EVALUATION AND COMPARISON OF HYDATID CYST FLUID AND PROTOSCOLEX ANTIGENS TO DIFFERENT INTERMEDIATE HOSTS AND HUMAN BY SDS-PAGE GEL ELECTROPHORESIS AND WESTERN BLOTTING. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2005;13(1):3-12. Available from: https://sid.ir/paper/90238/en

IEEE: Copy

A. RAFIEI, M. DEZFULINEJAD, and ASAD MIRZAEI, “EVALUATION AND COMPARISON OF HYDATID CYST FLUID AND PROTOSCOLEX ANTIGENS TO DIFFERENT INTERMEDIATE HOSTS AND HUMAN BY SDS-PAGE GEL ELECTROPHORESIS AND WESTERN BLOTTING,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 13, no. 1, pp. 3–12, 2005, [Online]. Available: https://sid.ir/paper/90238/en

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