ANALYSIS AND COMPARISON OF BIOCHEMICAL AND MOLECULAR STRUCTURAL CHARACTERISTICSAND PHYLOGENETIC TREND OF TWO H-TYPE THIOREDOXIN ISOFORMS FROM GRAPE (VITISVINIFERA L. CV. ASKARI)

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HEIDARI JAPELAGHI R.; HADDAD R.; GAROUSI GH.A.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2010 | Volume:2 | Issue:1 Page(s): 25-43

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Title

ANALYSIS AND COMPARISON OF BIOCHEMICAL AND MOLECULAR STRUCTURAL CHARACTERISTICSAND PHYLOGENETIC TREND OF TWO H-TYPE THIOREDOXIN ISOFORMS FROM GRAPE (VITISVINIFERA L. CV. ASKARI)

Author(s)

HEIDARI JAPELAGHI R. | HADDAD R. | GAROUSI GH.A. | Issue Writer Certificate

Keywords

BIOCHEMICAL CHARACTERISTICSQ2

CLONINGQ2

GRAPEQ3

PHYLOGENETICQ2

THIOREDOXINQ2

Abstract

Thioredoxins (Trxs) are small heat-stable proteins that participate in dithiol-disulfideexchange reactions. In contrast to other organisms, plants contain six different Trx types: f, m, x, y, o and h. The h-type Trx consists of multiple forms that involved in different processes such as cellular protection against oxidative, biotic and abiotic stresses. Two THIOREDOXIN h-type genes, called VvTrxh4 and VvCxxS2, were isolated of GRAPE (Vitisvinifera L. cv. Askari) berry tissue and were cloned into pUC19 plasmid vector. Reverse transcription was carried out using Polymerase Chain Reaction (RT-PCR), to analyze biochemical, structural and PHYLOGENETIC characteristics. Nucleotide sequence analysis revealed that the open reading frame of VvTrxh4 and VvCxxS2 genes are 345 bp and 381bp long, respectively and encode for proteins of 114 and 126 amino acid residues, respectively. Protein sequence analysis showed that VvTrxh4 gene contains a typical catalytic site WCGPC, whereas VvCxxS2 gene harbors the non-typical active site WCIPS. The calculated molecular mass and the predicted isoelectric point of the deduced polypeptides for both VvTrxh4 and VvCxxS2 were 12.76 kDa and 5.22, and also 14.25kDa and 4.68, respectively. Structural analysis showed that deduced proteins contain aidentical 3D structure, whereas PHYLOGENETIC study of cloned genes with THIOREDOXIN from other plants revealed that these genes are belonging to different subgroups. In the present research project, VvTrxh4 gene belongs to class IA, while VvCxxS2 gene belongs to class IIIB from h-type THIOREDOXINs.

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APA: Copy

HEIDARI JAPELAGHI, R., HADDAD, R., & GAROUSI, GH.A.. (2010). ANALYSIS AND COMPARISON OF BIOCHEMICAL AND MOLECULAR STRUCTURAL CHARACTERISTICSAND PHYLOGENETIC TREND OF TWO H-TYPE THIOREDOXIN ISOFORMS FROM GRAPE (VITISVINIFERA L. CV. ASKARI). JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 2(1), 25-43. SID. https://sid.ir/paper/224391/en

Vancouver: Copy

HEIDARI JAPELAGHI R., HADDAD R., GAROUSI GH.A.. ANALYSIS AND COMPARISON OF BIOCHEMICAL AND MOLECULAR STRUCTURAL CHARACTERISTICSAND PHYLOGENETIC TREND OF TWO H-TYPE THIOREDOXIN ISOFORMS FROM GRAPE (VITISVINIFERA L. CV. ASKARI). JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2010;2(1):25-43. Available from: https://sid.ir/paper/224391/en

IEEE: Copy

R. HEIDARI JAPELAGHI, R. HADDAD, and GH.A. GAROUSI, “ANALYSIS AND COMPARISON OF BIOCHEMICAL AND MOLECULAR STRUCTURAL CHARACTERISTICSAND PHYLOGENETIC TREND OF TWO H-TYPE THIOREDOXIN ISOFORMS FROM GRAPE (VITISVINIFERA L. CV. ASKARI),” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 2, no. 1, pp. 25–43, 2010, [Online]. Available: https://sid.ir/paper/224391/en

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