IDENTIFICATION, CLONING AND CHARACTERIZATION OF A THIOREDOXIN H (VVTRXH10) GENE ISOLATED FROM GRAPE (VITIS VINIFERA L.) CV. YAQUTI BERRY TISSUE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MUSSAVI S.S.; HADDAD R.; GAROUSI GH.; HOSSEINI R.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Agricultural Biotechnology Year:2010 | Volume:9 | Issue:1 Page(s): 17-26

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

745

Download:

223

Cites:

Information Journal Paper

Title

IDENTIFICATION, CLONING AND CHARACTERIZATION OF A THIOREDOXIN H (VVTRXH10) GENE ISOLATED FROM GRAPE (VITIS VINIFERA L.) CV. YAQUTI BERRY TISSUE

Author(s)

MUSSAVI S.S. | HADDAD R. | GAROUSI GH. | HOSSEINI R. | Issue Writer Certificate

Keywords

GRAPEVINEQ2

THIOREDOXIN HQ2

CLONINGQ2

SEQUENCE ANALYSISQ3

YAQUTI CULTIVARQ3

Abstract

Total RNA was extracted from grape (Vitis vinifera L.) cv. Yaquti berry tissue to characterize a THIOREDOXIN H gene (VvTrxh10). A cDNA library was synthesized using reverse transcription polymerase chain reaction (RT-PCR). Then, theVvTrxh10 gene was amplified, isolated and cloned in a pUC19 vector plasmid. Nucleotide SEQUENCE ANALYSIS revealed that the cloned cDNA expressed thioredoxin and contained a single open reading frame of 345 bp encoding a protein of 114 amino acid residues. Predicted protein SEQUENCE ANALYSIS showed that this gene contains a nongeneral catalic site RCGLC, characteristic tryptophan (W) and potential structural motif involving cell-to-cell taransfer (MAEE) in N-terminal. Phylogenetic and alignment studies revealed that such isoform belongs to the subgroup I fromh thioredoxins group. Moreovere, relevant predicted protein exhibited a high similarity with the other plant thioredoxinsh gene in the NCBI gene bank.

Cites

CLONING AND ANALYSIS OF PHYSICO-CHEMICAL AND STRUCTURAL CHARACTERISTICS OF A H-TYPE THIOREDOXIN GENE, BELONGING TO SUBGROUP I FROM GRAPE (VITIS VINIFERA L. CV. ASKARI) BERRY TISSUE

References

No record.

Cite

APA: Copy

MUSSAVI, S.S., HADDAD, R., GAROUSI, GH., & HOSSEINI, R.. (2010). IDENTIFICATION, CLONING AND CHARACTERIZATION OF A THIOREDOXIN H (VVTRXH10) GENE ISOLATED FROM GRAPE (VITIS VINIFERA L.) CV. YAQUTI BERRY TISSUE. AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH), 9(1), 17-26. SID. https://sid.ir/paper/219868/en

Vancouver: Copy

MUSSAVI S.S., HADDAD R., GAROUSI GH., HOSSEINI R.. IDENTIFICATION, CLONING AND CHARACTERIZATION OF A THIOREDOXIN H (VVTRXH10) GENE ISOLATED FROM GRAPE (VITIS VINIFERA L.) CV. YAQUTI BERRY TISSUE. AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH)[Internet]. 2010;9(1):17-26. Available from: https://sid.ir/paper/219868/en

IEEE: Copy

S.S. MUSSAVI, R. HADDAD, GH. GAROUSI, and R. HOSSEINI, “IDENTIFICATION, CLONING AND CHARACTERIZATION OF A THIOREDOXIN H (VVTRXH10) GENE ISOLATED FROM GRAPE (VITIS VINIFERA L.) CV. YAQUTI BERRY TISSUE,” AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH), vol. 9, no. 1, pp. 17–26, 2010, [Online]. Available: https://sid.ir/paper/219868/en

Related Journal Papers