EFFECTIVE FACTORS IN COTTON (GOSSIPIUM SPP) TRANSFORMATION USING AGROBACTERIUM

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TOHIDFAR M.; MOHSENPOUR M.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2010 | Volume:2 | Issue:1 Page(s): 1-24

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Title

EFFECTIVE FACTORS IN COTTON (GOSSIPIUM SPP) TRANSFORMATION USING AGROBACTERIUM

Author(s)

TOHIDFAR M. | MOHSENPOUR M. | Issue Writer Certificate

Keywords

AGROBACTERIUMQ2

COTTONQ2

TRANSFORMATIONQ3

Abstract

Cotton (Gossipium spp) is ranked first among the fibers producing plants in the world and is also considered as an important source of oil. Although significant progress has been made in traditional COTTON breeding programs, but there are still limitations including limited gene resources, lack of crosses, lack of efficient selection and high time consumption. Through recent advances in gene TRANSFORMATION to COTTON, not only facilitated TRANSFORMATION of useful genes has become possible, but also promising results have been obtained in gene identification as well as function and regulation studies. AGROBACTERIUM mediated TRANSFORMATION is one of the most used TRANSFORMATION methods in plants, including COTTON. Several factors could affect TRANSFORMATION efficiency such as bacterial strains and concentrations, added phenolic regent in plant culture medium, plant species and genotypes, use of plant growth regulators, explants type, light and temperature during co-cultivation, antibiotics, wounding the target tissue and suitable method for selection of transgenic cells. Hypocotyls, cotyledons and cell suspensions are used as explants in COTTON TRANSFORMATION using AGROBACTERIUM. These explants have some limitations such as low regeneration rate and genotype dependence. Therefore, regeneration could be obtained in only a limited number of species using these explants. On the other hand, establishment of a regeneration system using shoot apex meristem in COTTON has increased its TRANSFORMATION efficiency. This article is trying to review the factors shown to be effective in gene TRANSFORMATION into COTTON.

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APA: Copy

TOHIDFAR, M., & MOHSENPOUR, M.. (2010). EFFECTIVE FACTORS IN COTTON (GOSSIPIUM SPP) TRANSFORMATION USING AGROBACTERIUM. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 2(1), 1-24. SID. https://sid.ir/paper/224393/en

Vancouver: Copy

TOHIDFAR M., MOHSENPOUR M.. EFFECTIVE FACTORS IN COTTON (GOSSIPIUM SPP) TRANSFORMATION USING AGROBACTERIUM. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2010;2(1):1-24. Available from: https://sid.ir/paper/224393/en

IEEE: Copy

M. TOHIDFAR, and M. MOHSENPOUR, “EFFECTIVE FACTORS IN COTTON (GOSSIPIUM SPP) TRANSFORMATION USING AGROBACTERIUM,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 2, no. 1, pp. 1–24, 2010, [Online]. Available: https://sid.ir/paper/224393/en

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