CALLUS INDUCTION AND DIRECT SHOOT REGENERATION IN LEPIDIUM DRABA L.EXPLANTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ZINHARI Z.; POURSEYEDI SH.; ZOLALA J.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2016 | Volume:8 | Issue:2 Page(s): 31-51

Download Full-Text

Persian Verion

View:

1,440

Download:

Cites:

Information Journal Paper

Title

CALLUS INDUCTION AND DIRECT SHOOT REGENERATION IN LEPIDIUM DRABA L.EXPLANTS

Author(s)

ZINHARI Z. | POURSEYEDI SH. | ZOLALA J. | Issue Writer Certificate

Keywords

CALLUS INDUCTIONQ2

DIRECT SHOOT REGENERATIONQ2

LEPIDIUM DRABAQ2

TISSUE CULTURE OPTIMIZATIONQ1

Abstract

Lepidium drabathrough the production of anti-cancer and anti-microbial compound, sulforaphane, is considered a valuable medicinal plants.Therefore, optimization of in vitro tissue culture to facilitate genetic transformation and improvement of traits in this plant is important. This study was conducted, by cotyledon, stem and root explants inMSmedium in the form of two separate tests. Data were analyzed in a factorial experiment based on completely randomized design. In the first experiment with different concentrations of growth regulators NAA and BAP, the highest response rate, DIRECT SHOOT REGENERATION rate and direct shoot generation were related to root explants. The highest root production rate and root generation were showed in cotyledons explants.1 mgl-1 BAP without NAA induced the highest direct shoot generation in root explants. Cotyledons explants in 2 mgl-1 NAA and 0.5 mgl-1 BAP produced the highest Root production rate and the highest root generation. CALLUS INDUCTION rate and callus initiation of stem explants were more than others, but the callus regeneration rate in three studied explants showed no significant difference. The highest shoot generation from regenerated callus was observed in the cotyledons explants. The second experiment with a combination of different concentrations of 2, 4-D and BAP, under the influence of two treatments darkness and 16h photoperiod was performed. The highest investigated traits was in the 16h photoperiod. Cotyledons and stem explants were showed the highest CALLUS INDUCTION rate and leaf explants showed the greatest weight of callus; While the highest callus regeneration rate and callus growth rate were obtained from the root explants. From leaf explants, the highest callus weightwas obtained from 0.5 mgl-1 2, 4-D with 3 mgl-1BAP and the most growth rate of callus was obtained from 0.5 and 1 mgl-1 2, 4-D and 3 mgl-1BAP. Also the combination of 2 mgl-1 2, 4-D with 2 mgl-1 BAP and 3 mgl-1 BAP, produced the highest callus regeneration in root explants.

Cites

No record.

References

No record.

Cite

APA: Copy

ZINHARI, Z., POURSEYEDI, SH., & ZOLALA, J.. (2016). CALLUS INDUCTION AND DIRECT SHOOT REGENERATION IN LEPIDIUM DRABA L.EXPLANTS. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 8(2), 31-51. SID. https://sid.ir/paper/224520/en

Vancouver: Copy

ZINHARI Z., POURSEYEDI SH., ZOLALA J.. CALLUS INDUCTION AND DIRECT SHOOT REGENERATION IN LEPIDIUM DRABA L.EXPLANTS. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2016;8(2):31-51. Available from: https://sid.ir/paper/224520/en

IEEE: Copy

Z. ZINHARI, SH. POURSEYEDI, and J. ZOLALA, “CALLUS INDUCTION AND DIRECT SHOOT REGENERATION IN LEPIDIUM DRABA L.EXPLANTS,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 8, no. 2, pp. 31–51, 2016, [Online]. Available: https://sid.ir/paper/224520/en

Related Journal Papers