Callus Induction and Plant Regeneration of Chrysanthemum morifoliumand C. coccineumvia Direct and Indirect Organogenesis and Genetic Fidelity Analysis Using IRAP, ISSR and SCoT Molecular Markers

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NASRI FARDIN; ZAKIZADEH HEDAYAT; VAFAEE YAVAR; MOZAFARI ALI AKBAR

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Journal: JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS) Year:2018 | Volume:8 | Issue:4 Page(s): 265-284

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Title

Callus Induction and Plant Regeneration of Chrysanthemum morifoliumand C. coccineumvia Direct and Indirect Organogenesis and Genetic Fidelity Analysis Using IRAP, ISSR and SCoT Molecular Markers

Author(s)

NASRI FARDIN | ZAKIZADEH HEDAYAT | VAFAEE YAVAR | MOZAFARI ALI AKBAR | Issue Writer Certificate

Keywords

Callus inductionQ2

Cultivar responseQ1

Direct regenerationQ2

EmbryogenesisQ2

Genetic fidelityQ1

Abstract

In vitro propagation of C. morifolium cv. ‘ Homa’ and cv. ‘ Delkash’ and wild C. coccineum via direct and indirect organogenesis and somatic Embryogenesis were investigated. BAP at 0, 1, 2 and 3 mg l-1 or NAA at 0, 0. 05, 0. 1 and 0. 2 mg l-1 concentrations were used to induce direct and indirect organogenesis of shoot tip explants. To study the Callus induction and somatic Embryogenesis, the young leaf explants were cultured on MS medium containing BAP (0, 1, 2 or 3 mg l-1) and 2, 4-D (0, 1, 2 or 3 mg l-1). Direct shoot regeneration was achieved from shoot tip explants of ‘ Homa’ and ‘ Delkash’ as well as C. coccineum. The highest number of shoots through Direct regeneration (13. 78 and 8. 89 shoots per explant for C. coccineum and C. morifoilum ‘ Homa’ , respectively) were observed in the treatment with 2 mg l-1 BAP and 0. 05 mg l-1 NAA. In both species, the highest frequency of callus formation and Embryogenesis were obtained on medium containing 2. 0 mg l-1 2, 4-D and 2 mg l-1 BAP. Genetic fidelity of 10 acclimatized plants derived from Direct regeneration of each species was confirmed using six inter-retrotransposon amplified polymorphism (IRAP), inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) primers. A total of 56, 56 and 39 fragments were amplified for IRAP, ISSR, and SCoT, respectively. In general, our results showed that finding a better response of explants to Embryogenesis or organogenesis in a specific cultivar and with special PGRs combinations and concentrations play an important role in the in vitro propagation efficiency of chrysanthemum species.

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APA: Copy

NASRI, FARDIN, ZAKIZADEH, HEDAYAT, VAFAEE, YAVAR, & MOZAFARI, ALI AKBAR. (2018). Callus Induction and Plant Regeneration of Chrysanthemum morifoliumand C. coccineumvia Direct and Indirect Organogenesis and Genetic Fidelity Analysis Using IRAP, ISSR and SCoT Molecular Markers. JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS), 8(4 ), 265-284. SID. https://sid.ir/paper/242584/en

Vancouver: Copy

NASRI FARDIN, ZAKIZADEH HEDAYAT, VAFAEE YAVAR, MOZAFARI ALI AKBAR. Callus Induction and Plant Regeneration of Chrysanthemum morifoliumand C. coccineumvia Direct and Indirect Organogenesis and Genetic Fidelity Analysis Using IRAP, ISSR and SCoT Molecular Markers. JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS)[Internet]. 2018;8(4 ):265-284. Available from: https://sid.ir/paper/242584/en

IEEE: Copy

FARDIN NASRI, HEDAYAT ZAKIZADEH, YAVAR VAFAEE, and ALI AKBAR MOZAFARI, “Callus Induction and Plant Regeneration of Chrysanthemum morifoliumand C. coccineumvia Direct and Indirect Organogenesis and Genetic Fidelity Analysis Using IRAP, ISSR and SCoT Molecular Markers,” JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS), vol. 8, no. 4 , pp. 265–284, 2018, [Online]. Available: https://sid.ir/paper/242584/en

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