HETEROLOGOUS EXPRESSION OF A RICE METALLOTHIONEIN TYPE 1 ISOFORM IN ESHERICHIA COLI AND STUDY OF IT’S BINDING ABILITY TO NICKEL

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MOHAMMADI NEZHAD R.; SHAHPIRI A.; MIRLOHI A.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2014 | Volume:6 | Issue:1 Page(s): 163-181

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Information Journal Paper

Title

HETEROLOGOUS EXPRESSION OF A RICE METALLOTHIONEIN TYPE 1 ISOFORM IN ESHERICHIA COLI AND STUDY OF IT’S BINDING ABILITY TO NICKEL

Author(s)

MOHAMMADI NEZHAD R. | SHAHPIRI A. | MIRLOHI A. | Issue Writer Certificate

Keywords

CLONINGQ1

HEAVY METALSQ2

HETEROLOGOUS PROTEIN EXPRESSIONQ2

METALLOTHIONEINQ2

Abstract

Plants process several potential cellular mechanisms for detoxification of HEAVY METALS. One of the most important mechanisms is synthesis of metal binding peptides and proteins such as METALLOTHIONEINs (MTs). MTs are low molecular weight and cystein-rich proteins that can bind metal ions through their thiol groups. In this study the coding sequence of gene encoding OsMTI-1b isoforms from rice (), was cloned in pET41a and transferred into expression host, Escherichia coli strain Rosetta (DE3). After induction with IPTG, considerable amount of recombinant proteins was produced in the soluble fraction of the E.coli transforman. Recombinant proteins were purified using affinity chromatography. The tolerance of cells expressing recombinant proteins toward Ni, were compared to control by plotting their growth curve in addition to determination of the amount of accumulated Ni ions in bacterial cells and culture medium using inductively coupled plasma atomic emission spectroscopy (ICP-AES). According to the results, over-expression of OsMTI-1b isoform increased the tolerance of E. coli cells to Ni through accumulation of more metal ions inside cells. Furthermore, the UV absorption spectra and competitive reactions of in vitro Niincubated proteins with 5-5' dithiobis (2-nitrobenzoic) acid (DTNB) revealed that GSTOsMTI- 1b protein is able to form Ni-thiolate clusters. Taken together, these data indicate that OsMTI-1b isoform may be involved in protection of rice cells against heavy metal toxicity.

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APA: Copy

MOHAMMADI NEZHAD, R., SHAHPIRI, A., & MIRLOHI, A.. (2014). HETEROLOGOUS EXPRESSION OF A RICE METALLOTHIONEIN TYPE 1 ISOFORM IN ESHERICHIA COLI AND STUDY OF IT’S BINDING ABILITY TO NICKEL. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 6(1), 163-181. SID. https://sid.ir/paper/224541/en

Vancouver: Copy

MOHAMMADI NEZHAD R., SHAHPIRI A., MIRLOHI A.. HETEROLOGOUS EXPRESSION OF A RICE METALLOTHIONEIN TYPE 1 ISOFORM IN ESHERICHIA COLI AND STUDY OF IT’S BINDING ABILITY TO NICKEL. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2014;6(1):163-181. Available from: https://sid.ir/paper/224541/en

IEEE: Copy

R. MOHAMMADI NEZHAD, A. SHAHPIRI, and A. MIRLOHI, “HETEROLOGOUS EXPRESSION OF A RICE METALLOTHIONEIN TYPE 1 ISOFORM IN ESHERICHIA COLI AND STUDY OF IT’S BINDING ABILITY TO NICKEL,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 6, no. 1, pp. 163–181, 2014, [Online]. Available: https://sid.ir/paper/224541/en

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