Investigating Genetic and Phytochemical Diversity of Cumin Ecotypes Cultivated in Different Rangelands of Yazd Province

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HOSSEIN JAFARI SAMIRA; SAADATFAR AMIR; MOHKAMI AFSANEH; KARIMIAN ALI AKBAR

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2020 | Volume:11 | Issue:4 Page(s): 51-66

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Title

Investigating Genetic and Phytochemical Diversity of Cumin Ecotypes Cultivated in Different Rangelands of Yazd Province

Author(s)

HOSSEIN JAFARI SAMIRA | SAADATFAR AMIR | MOHKAMI AFSANEH | KARIMIAN ALI AKBAR | Issue Writer Certificate

Keywords

Cluster analysisQ1

Genetic diversityQ2

ISSR markerQ1

Medicinal plantQ2

PhytochemistryQ2

Abstract

Objective According to economic value of cumin (Cuminum cyminum) Medicinal plant, it is essential to do its genetic and phytochemical assessment to conserve genetic sources and achieve better quality of essential oil in breeding programs. This study purpose is to investigate genetic and phytochemical diversity of different cumin ecotypes in Yazd province. Materials and methods To study Genetic diversity using 10 ISSR markers, cumin plants DNA extraction of four habitats, was done using CTAB method. The quantity and quality of DNA were determined using spectrophotometer and electrophoresis. Clear Bands resulting from electrophoresis of PCR products on 1. 5% agarose gel were scored. To investigate phytochemical diversity using GC-MS method, essential oil from cumin seeds was extracted via water steam distillation method. Results The results showed that ISSR-14 and ISSR-13 primers had higher amount of PIC (0. 39 and 0. 38) and MI (13. 3 and 12. 48) and number of bands (35 and 32). Cluster analysis using Jacquard's coefficient and UPGMA method, grouped four populations into 8 groups. PCA analysis showed three components explained 57. 17% of total variance. The results of this analysis correspond with Cluster analysis and match with the geographical regions. Analysis of molecular variance showed genetic variation within and among populations 93% and 7% of total variation, respectively. On the basis of GC-MS results, Propanal, Benzenmethanol, 1-phenyl-1-butanol, γ-terpinene, β-Pinene and P-cymene compounds were identified as the main compounds. Cluster analysis of samples on the basis of phytochemical parameters put Taft, Mehriz and Ardakan habitats in one group and Bahabad habitat in another group so that it doesn’ t correspond with geographical distance. Conclusions Based the results, it can be concluded that there is high potential of diversity among cumin ecotypes. ISSR application in assessing Genetic diversity of the ecotypes was successful. Grouping on the basis of phytochemical compounds didn’ t correspond with genetic grouping.

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APA: Copy

HOSSEIN JAFARI, SAMIRA, SAADATFAR, AMIR, MOHKAMI, AFSANEH, & KARIMIAN, ALI AKBAR. (2020). Investigating Genetic and Phytochemical Diversity of Cumin Ecotypes Cultivated in Different Rangelands of Yazd Province. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 11(4 ), 51-66. SID. https://sid.ir/paper/224555/en

Vancouver: Copy

HOSSEIN JAFARI SAMIRA, SAADATFAR AMIR, MOHKAMI AFSANEH, KARIMIAN ALI AKBAR. Investigating Genetic and Phytochemical Diversity of Cumin Ecotypes Cultivated in Different Rangelands of Yazd Province. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2020;11(4 ):51-66. Available from: https://sid.ir/paper/224555/en

IEEE: Copy

SAMIRA HOSSEIN JAFARI, AMIR SAADATFAR, AFSANEH MOHKAMI, and ALI AKBAR KARIMIAN, “Investigating Genetic and Phytochemical Diversity of Cumin Ecotypes Cultivated in Different Rangelands of Yazd Province,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 11, no. 4 , pp. 51–66, 2020, [Online]. Available: https://sid.ir/paper/224555/en

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