BIOINFORMATICS ANALYSES OF EXPRESSED SEQUENCE TAGS IN CHINESE SPRING WHEAT SPIKES UNDER SALT STRESS

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ZINATI Z.; ALEMZADEH A.; EBRAHIMIE E.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2016 | Volume:8 | Issue:3 Page(s): 34-49

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Title

BIOINFORMATICS ANALYSES OF EXPRESSED SEQUENCE TAGS IN CHINESE SPRING WHEAT SPIKES UNDER SALT STRESS

Author(s)

ZINATI Z. | ALEMZADEH A. | EBRAHIMIE E. | Issue Writer Certificate

Keywords

SALT STRESSQ2

FUNCTIONAL GENOMICSQ2

SPIKEQ2

Abstract

Functional genomics helps to understand the key mechanisms involved in SALT STRESStoleranceandallows the possibility of targeted genetic manipulation to improve crop tolerance tosalinity . The goal of this study was to identify new genes related to SALT STRESS and functional analysis based on expressed sequence tags (EST). Therefore, two EST libraries under control and SALT STRESSed conditions were downloaded from Harvard and Graingenes databanks. EGassembler, NCBI BLAST, MaxPlanck and IDEG6 software tools were applied for clustering and assembling EST sequences, determination of proteins related to unigenes (contigs and singletons), functional categories and statistics test, respectively. Results showed that twenty functional categories are significantly different between control and stress conditions. Most of contigs and singletons fell into protein and RNA categories whereas CHO metabolism and TCA and organic transformation had the minimum of numbers of contigs and singletons in both control and SALT STRESSed conditions. Percentage of unigenes in mitochondrial electron transport/ATP synthesis, Lipid metabolism, redox, protein, RNA, DNA, and cell categories were significantly higher in stress condition compared with control condition. Therefore, these functional categories could be involved in the mechanism ofresponse to SALT STRESS. Two hundred and seventy-one genes were differentially expressed that grouped into 23 functional categories. The wheat genes identified in this study can be considered as candidates genes to improve salt tolerance through genetic manipulation.

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APA: Copy

ZINATI, Z., ALEMZADEH, A., & EBRAHIMIE, E.. (2016). BIOINFORMATICS ANALYSES OF EXPRESSED SEQUENCE TAGS IN CHINESE SPRING WHEAT SPIKES UNDER SALT STRESS. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 8(3), 34-49. SID. https://sid.ir/paper/224564/en

Vancouver: Copy

ZINATI Z., ALEMZADEH A., EBRAHIMIE E.. BIOINFORMATICS ANALYSES OF EXPRESSED SEQUENCE TAGS IN CHINESE SPRING WHEAT SPIKES UNDER SALT STRESS. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2016;8(3):34-49. Available from: https://sid.ir/paper/224564/en

IEEE: Copy

Z. ZINATI, A. ALEMZADEH, and E. EBRAHIMIE, “BIOINFORMATICS ANALYSES OF EXPRESSED SEQUENCE TAGS IN CHINESE SPRING WHEAT SPIKES UNDER SALT STRESS,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 8, no. 3, pp. 34–49, 2016, [Online]. Available: https://sid.ir/paper/224564/en

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