STUDY ON THE ROOT ADVENTITIOUS ROOT FORMATION IN SOME HORTICULTURAL CROPS USING AGROBACTERIUM RHIZOGENESE

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AZIZI M.; AGHABOZORGEE M.; FARSEE M.; TEHRANIFAR A.; ZOLALLA J.; GHABOOLIL M.

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Journal: Agricultural Sciences and Technology Year:2007 | Volume:21 | Issue:2 (SPECIAL ISSUE IN HORTICULTURE SCIENCES) Page(s): 79-90

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Title

STUDY ON THE ROOT ADVENTITIOUS ROOT FORMATION IN SOME HORTICULTURAL CROPS USING AGROBACTERIUM RHIZOGENESE

Author(s)

Keywords

AGROBACTERIUM RHIZOGENESQ3

A4 AND GMI9534Q3

ADVENTITIOUS ROOTQ3

BERBERIS VULGAR VAR. ASPERN1AQ3

LAVANDULA SPICAQ3

FICUS BENJAMINE AND ROSA SPP.Q3

Abstract

One of the main obstacles in vegetative propagation of plants is hard root formation. A. rhizogenes mediated transformation dissolved the problem and could be used as an alternative root induction method. In this research, we used two strains of A. rhizogenes (A4 and GMI9534) for root induction and transformed root production of three stem type (current season growth, 1-2 years old and 5-6 years old stem) in some horticultural crops. This study was conducted in two factorial experiments in randomized complete block design. In first experiment (in vitro) we inoculated shoots of Berberis vulgar var. asperma and sterile seedling of LAVANDULA SPICA. In second experiment (In vivo) we inoculated stem cutting (Berberis vulgar var. asperma, Ficus benjamine) and air layered stem of Berberis vulgar var. asperma, Ficus benjamine, Rosa spp, Rosmarinus officinal is with A. rhizogenes for root induction. Results showed that GMI9534 increased root induction of sterile seedling of LAVANDULA SPICA significantly (p<0.01) in comparison with A4 and control. Hardwood stem (1-2 years old) of Rosmarinus officinal is that inoculated with GMI9534 significantly (p<0.05) produced more root than A4 and control treatment. Root induction of inoculated stem cutting and layerage (1- 2 years old) in FICUS BENJAMINE AND ROSA SPP.with GMI9534 was significantly (p<0.01) higher than A4 and control. PCR analysis of DNA extracted from transformed root, confirmed that rolC gene has been transferred to ADVENTITIOUS ROOTs by A. rhizogenes.

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APA: Copy

AZIZI, M., AGHABOZORGEE, M., FARSEE, M., TEHRANIFAR, A., ZOLALLA, J., & GHABOOLIL, M.. (2007). STUDY ON THE ROOT ADVENTITIOUS ROOT FORMATION IN SOME HORTICULTURAL CROPS USING AGROBACTERIUM RHIZOGENESE. AGRICULTURAL SCIENCES AND TECHNOLOGY, 21(2 (SPECIAL ISSUE IN HORTICULTURE SCIENCES)), 79-90. SID. https://sid.ir/paper/22473/en

Vancouver: Copy

AZIZI M., AGHABOZORGEE M., FARSEE M., TEHRANIFAR A., ZOLALLA J., GHABOOLIL M.. STUDY ON THE ROOT ADVENTITIOUS ROOT FORMATION IN SOME HORTICULTURAL CROPS USING AGROBACTERIUM RHIZOGENESE. AGRICULTURAL SCIENCES AND TECHNOLOGY[Internet]. 2007;21(2 (SPECIAL ISSUE IN HORTICULTURE SCIENCES)):79-90. Available from: https://sid.ir/paper/22473/en

IEEE: Copy

M. AZIZI, M. AGHABOZORGEE, M. FARSEE, A. TEHRANIFAR, J. ZOLALLA, and M. GHABOOLIL, “STUDY ON THE ROOT ADVENTITIOUS ROOT FORMATION IN SOME HORTICULTURAL CROPS USING AGROBACTERIUM RHIZOGENESE,” AGRICULTURAL SCIENCES AND TECHNOLOGY, vol. 21, no. 2 (SPECIAL ISSUE IN HORTICULTURE SCIENCES), pp. 79–90, 2007, [Online]. Available: https://sid.ir/paper/22473/en

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