MOLECULAR COMPARISON OF GENOMIC 5' REGION OF MAIZE DWARF MOSAIC VIRUS (MDMV) GOLESTAN ISOLATE WITH OTHER CEREAL POTYVIRUSES

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MOSTAFAVI NEISHABURI F.S.; NASROLAHNEJAD S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Crop Biotechnology Year:2013 | Volume:2 | Issue:3 Page(s): 25-33

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Title

MOLECULAR COMPARISON OF GENOMIC 5' REGION OF MAIZE DWARF MOSAIC VIRUS (MDMV) GOLESTAN ISOLATE WITH OTHER CEREAL POTYVIRUSES

Author(s)

MOSTAFAVI NEISHABURI F.S. | NASROLAHNEJAD S. | Issue Writer Certificate

Keywords

SEQUENCESQ2

GENOMEQ2

FEATURE MOLECULARQ2

MAIZE DWARF MOSAIC VIRUSQ2

Abstract

Maize dwarf mosaic virus is one of the most important and most extensive viruses of maize in the world. In this paper investigated molecular comparison and nucleotide sequence analysis of coding regions of proteins of genomic 5' region of Maize dwarf mosaic with other isolate and other cereal potyviruses. In order to this, maize plants with mosaic symptoms and dwarfing were collected from Golestan province. After verification of plants by Elisa test against MDMV antiserum, an infected sample was selected for isolation of total RNA. Amplification of genomic 5' region has been conducted using five specific primer pair that was obtained 5 overlap segments including P1, HC-Pro ,p3, 6k1genes, as well as 5'-UTR region of the GENOME and was obtained 3460bp. The comparative analysis of nucleotide SEQUENCES of MDMV isolates showed that the Iranian (Golestan) isolate have 95.7 and 92.3 percent similarity at the amino acid level and 90.5 and 85.1 percent at the nucleotide level with Bulgarian and Spanish isolates respectively, and with other homogeneous viruses, MDMV had similarity maximum with sorghum mosaic virus from Texas and sugarcane mosaic virus from china 72.9 and 72.3 percent respectively.

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APA: Copy

MOSTAFAVI NEISHABURI, F.S., & NASROLAHNEJAD, S.. (2013). MOLECULAR COMPARISON OF GENOMIC 5' REGION OF MAIZE DWARF MOSAIC VIRUS (MDMV) GOLESTAN ISOLATE WITH OTHER CEREAL POTYVIRUSES. JOURNAL OF CROP BIOTECHNOLOGY, 2(3), 25-33. SID. https://sid.ir/paper/226805/en

Vancouver: Copy

MOSTAFAVI NEISHABURI F.S., NASROLAHNEJAD S.. MOLECULAR COMPARISON OF GENOMIC 5' REGION OF MAIZE DWARF MOSAIC VIRUS (MDMV) GOLESTAN ISOLATE WITH OTHER CEREAL POTYVIRUSES. JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2013;2(3):25-33. Available from: https://sid.ir/paper/226805/en

IEEE: Copy

F.S. MOSTAFAVI NEISHABURI, and S. NASROLAHNEJAD, “MOLECULAR COMPARISON OF GENOMIC 5' REGION OF MAIZE DWARF MOSAIC VIRUS (MDMV) GOLESTAN ISOLATE WITH OTHER CEREAL POTYVIRUSES,” JOURNAL OF CROP BIOTECHNOLOGY, vol. 2, no. 3, pp. 25–33, 2013, [Online]. Available: https://sid.ir/paper/226805/en

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