STUDY OF REFERENCE GENES IN SESAME LEAVES UNDER SALT STRESS BY REAL-TIME PCR METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHAKERI SAMIRA; KAZEMITABAR SEYED KAMAL; HASHEMI SEYED HAMID REZA

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Crop Biotechnology Year:2015 | Volume:4 | Issue:8 Page(s): 1-10

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,034

Download:

Cites:

Information Journal Paper

Title

STUDY OF REFERENCE GENES IN SESAME LEAVES UNDER SALT STRESS BY REAL-TIME PCR METHOD

Author(s)

SHAKERI SAMIRA | KAZEMITABAR SEYED KAMAL | HASHEMI SEYED HAMID REZA | Issue Writer Certificate

Keywords

GENE EXPRESSIONQ1

SALT STRESSQ2

REFERENCE GENESQ2

SESAMUM INDICUM L.Q2

Abstract

Analysis of GENE EXPRESSION is considered as an essential part of functional genomics studies in all living organisms. Real-time PCR technique is very strong one to study the expression of a gene. However, despite its reliability, it has a set of specific problems, such as internal control gene selection which are suitable for normalization of the data. The study about selection reference of genes in sesame plant, at different developmental stages and under salinity stress of were studied. For this purpose, four internal control genes consists of eIF4- A, UBQ5, Alpha-Tubulin and Beta-Actin which are commonly used as housekeeping genes in plants, are selected and the stability of its expression in different salinity levels (zero and 75 mM) and different growth stages in five time periods (0 h, 6 h, 1 day, 4 days, 8 days and 16 days) in leaf tissue were examined. Study of the expression of REFERENCE GENES using geNORM software showed that, in developmental stages and salinity in the leaf tissues, eIF4-A and Beta-Actin genes have more stable expression than other investigated genes. Using these genes can be useful in normalization of GENE EXPRESSION by Real-Time PCR analysis. The results can be used as REFERENCE GENES for GENE EXPRESSION analysis in the Real-Time PCR.

Cites

No record.

References

No record.

Cite

APA: Copy

SHAKERI, SAMIRA, KAZEMITABAR, SEYED KAMAL, & HASHEMI, SEYED HAMID REZA. (2015). STUDY OF REFERENCE GENES IN SESAME LEAVES UNDER SALT STRESS BY REAL-TIME PCR METHOD. JOURNAL OF CROP BIOTECHNOLOGY, 4(8), 1-10. SID. https://sid.ir/paper/226822/en

Vancouver: Copy

SHAKERI SAMIRA, KAZEMITABAR SEYED KAMAL, HASHEMI SEYED HAMID REZA. STUDY OF REFERENCE GENES IN SESAME LEAVES UNDER SALT STRESS BY REAL-TIME PCR METHOD. JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2015;4(8):1-10. Available from: https://sid.ir/paper/226822/en

IEEE: Copy

SAMIRA SHAKERI, SEYED KAMAL KAZEMITABAR, and SEYED HAMID REZA HASHEMI, “STUDY OF REFERENCE GENES IN SESAME LEAVES UNDER SALT STRESS BY REAL-TIME PCR METHOD,” JOURNAL OF CROP BIOTECHNOLOGY, vol. 4, no. 8, pp. 1–10, 2015, [Online]. Available: https://sid.ir/paper/226822/en

Related Journal Papers