Analysis of the stability of housekeeping genes expression to study the gene expression in rice using by Real-time PCR

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EBRAHIMI AMIN; Amerian Mohammad Reza; Beyraghdar Kashkooli Arman

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Journal: Iranian Journal of Field Crop Science Year:2019 | Volume:49 | Issue:4 Page(s): 151-160

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Title

Analysis of the stability of housekeeping genes expression to study the gene expression in rice using by Real-time PCR

Author(s)

EBRAHIMI AMIN | Amerian Mohammad Reza | Beyraghdar Kashkooli Arman | Issue Writer Certificate

Keywords

Oryza sativaQ2

Real time PCRQ1

Salinity stressQ2

Reference gene and Best KeeperQ1

Abstract

A significant progress has been made in the expression profiling of living organism with invention of Real time PCR, because this method is one of the suitable methods for evaluating the gene expressions. In this method, it is crucial to control the error observed between samples. Normalization with a housekeeping gene is widely utilized to check the errors observed among samples in this method. In the current study, expression patterns of 10 housekeeping genes under Salinity stresses were evaluated. eight-week old plants were evaluated under Salinity stress at concentrations of 0, 200 and 400 Mm. Leaves and roots Samples were collected from the treated plants at 0, 6, 12, 24, 48 and 72 hour post treatment. Expression analysis of the obtained data was performed via geNorm software and it was shown that the eIF-4a as well as ACT7 genes in the root tissues and GAPDH and ACT11 in the leaf tissues was constitutively expressed. Based on the results of the analysis trough Best Keeper, the eIF-4a gene (in root) and UBQ10 and eIF-4a (in leaf) showed the highest correlation with the index of BestKeeper. Thus, it was concluded that the eIF-4a, ACT7, GAPDH, UBQ10 and ACT11 genes could be considered as suitable housekeeping genes to be used in the normalization of the derived from Rice.

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APA: Copy

EBRAHIMI, AMIN, Amerian, Mohammad Reza, & Beyraghdar Kashkooli, Arman. (2019). Analysis of the stability of housekeeping genes expression to study the gene expression in rice using by Real-time PCR. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 49(4 ), 151-160. SID. https://sid.ir/paper/374657/en

Vancouver: Copy

EBRAHIMI AMIN, Amerian Mohammad Reza, Beyraghdar Kashkooli Arman. Analysis of the stability of housekeeping genes expression to study the gene expression in rice using by Real-time PCR. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2019;49(4 ):151-160. Available from: https://sid.ir/paper/374657/en

IEEE: Copy

AMIN EBRAHIMI, Mohammad Reza Amerian, and Arman Beyraghdar Kashkooli, “Analysis of the stability of housekeeping genes expression to study the gene expression in rice using by Real-time PCR,” IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 49, no. 4 , pp. 151–160, 2019, [Online]. Available: https://sid.ir/paper/374657/en

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