CLONING AND EXPRESSION OF AN OMIGANAN PENTAHYDROCHLORIDE (MBI226) ANTIMICROBIAL PEPTIDE IN TOBACCO (NICOTIANA TABACUM) CONFERS RESIST TO BACTERIAL PATHOGENS

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BADRHADAD AZAM; NAZARIAN FIROUZABADI FARHAD; ISMAILI AHMAD; BAGHERI HEDAYAT

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Journal: Crop Biotechnology Year:2017 | Volume:7 | Issue:19 Page(s): 1-13

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Title

CLONING AND EXPRESSION OF AN OMIGANAN PENTAHYDROCHLORIDE (MBI226) ANTIMICROBIAL PEPTIDE IN TOBACCO (NICOTIANA TABACUM) CONFERS RESIST TO BACTERIAL PATHOGENS

Author(s)

BADRHADAD AZAM | NAZARIAN FIROUZABADI FARHAD | ISMAILI AHMAD | BAGHERI HEDAYAT | Issue Writer Certificate

Keywords

ANTIMICROBIAL PEPTIDESQ1

AGROBACTERIUM TUMEFACIENSQ1

PATHOGENQ1

RECOMBINANT PROTEINQ2

TOBACCOQ2

Abstract

ANTIMICROBIAL PEPTIDES are ancient and conserved molecules which are found in defense mechanisms of almost all living organisms from bacteria to animal and plant species. Identification and introduction of novel ANTIMICROBIAL PEPTIDES, is a cost-effective way to improve crop plants resistance to PATHOGENs by using recombinant DNA technology. Therefore, an expression construct containing omiganan antimicrobial encoding gene from the cytoplasmic granules of bovine neutrophils, was cloned and transferred to the TOBACCO leaf disk using AGROBACTERIUM TUMEFACIENSmediated-transformation. The presence of the antimicrobial peptide encoding gene in the genome of transgenic plants was confirmed by PCR analysis. Six putative transgenic lines and a non-transgenic control plant were selected for further molecular analysis. Total protein was extract from transgenic and nontransgenic control plants and used for antimicrobial activity assay against some human; E. coli, Salmonella, Staphylococcus, Bacillus, and plant: Xanthomonas and Pseudomonaspathogens by disc diffusion method. Results of this experiment showed that total protein extract from transgenic lines, as compared to non-transgenic plant, was significantly (P<0.05) able to inhibit bacteria growth. There was a significant difference (P<0.05) between transgenic lines with respect to anti-bacteria effect. In contrary to gram-negative bacteria, omiganan peptide did not have a significant effect (P>0.05) on gram-positive PATHOGENic bacteria. Therefore, it is possible to express and purify omiganan peptide from transgenic and used it in pharmacology formulations as an anti-bacterial medicine. Furthermore, this approach may offer an opportunity to generate transgenic crop plants expressing omiganan peptide resistance to some devastating plant bacteria PATHOGENs.

Cites

Cloning and in planta expression of an omiganan antimicrobial peptide in tobacco and potato plants to control the growth of human bacterial pathogens

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APA: Copy

BADRHADAD, AZAM, NAZARIAN FIROUZABADI, FARHAD, ISMAILI, AHMAD, & BAGHERI, HEDAYAT. (2017). CLONING AND EXPRESSION OF AN OMIGANAN PENTAHYDROCHLORIDE (MBI226) ANTIMICROBIAL PEPTIDE IN TOBACCO (NICOTIANA TABACUM) CONFERS RESIST TO BACTERIAL PATHOGENS. JOURNAL OF CROP BIOTECHNOLOGY, 7(19 ), 1-13. SID. https://sid.ir/paper/226829/en

Vancouver: Copy

BADRHADAD AZAM, NAZARIAN FIROUZABADI FARHAD, ISMAILI AHMAD, BAGHERI HEDAYAT. CLONING AND EXPRESSION OF AN OMIGANAN PENTAHYDROCHLORIDE (MBI226) ANTIMICROBIAL PEPTIDE IN TOBACCO (NICOTIANA TABACUM) CONFERS RESIST TO BACTERIAL PATHOGENS. JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2017;7(19 ):1-13. Available from: https://sid.ir/paper/226829/en

IEEE: Copy

AZAM BADRHADAD, FARHAD NAZARIAN FIROUZABADI, AHMAD ISMAILI, and HEDAYAT BAGHERI, “CLONING AND EXPRESSION OF AN OMIGANAN PENTAHYDROCHLORIDE (MBI226) ANTIMICROBIAL PEPTIDE IN TOBACCO (NICOTIANA TABACUM) CONFERS RESIST TO BACTERIAL PATHOGENS,” JOURNAL OF CROP BIOTECHNOLOGY, vol. 7, no. 19 , pp. 1–13, 2017, [Online]. Available: https://sid.ir/paper/226829/en

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