Cloning and Expression of Two New Recombinant Antimicrobial Dermaseptin B1 Peptides in Tobacco to Control the Growth of Human Bacterial Pathogens

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Khademi Mitra; NAZARIAN FIROUZABADI FARHAD; ISMAILI AHMAD

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:29 | Issue:176 Page(s): 47-60

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

375

Download:

Cites:

Information Journal Paper

Title

Cloning and Expression of Two New Recombinant Antimicrobial Dermaseptin B1 Peptides in Tobacco to Control the Growth of Human Bacterial Pathogens

Author(s)

Khademi Mitra | NAZARIAN FIROUZABADI FARHAD | ISMAILI AHMAD | Issue Writer Certificate

Keywords

Agrobacterium rhizogenesQ1

gene expressionQ2

dermaseptin B1Q1

antimicrobialQ2

Abstract

Background and purpose: Rapid emergence of traditional antibiotic-resistant pathogens is one of the most important global challenges in medical sciences. To this end, substitution of current antibiotics with strong antimicrobial peptides could be of great benefit. Materials and methods: In this study, the DNA sequence encoding dermaseptin B1 (DrsB1) antimicrobial peptide derived from Phyllomedusa bicolor frog species was fused to either N or C terminal end of the sequence encoding the chitin-binding domain of the Avr4 gene from Cladosporium fulvum. The recombinant expression vectors containing two separate structures were transferred to Agrobacterium rhizogenes bacterium and then used to produce Hairy Roots (HRs) in tobacco plants. Recombinant dermaseptin B1 peptides were extracted from HRs and their antimicrobial activity was evaluated against some important human pathogens. Results: Transgene integration and expression of recombinant DrsB1 in hairy roots were confirmed by PCR and semi-quantitative RT-PCR analysis, respectively. antimicrobial activity of the protein extracts from transgenic HRs showed that both recombinant proteins had significant inhibitory effects on the bacterial pathogens growth (P<0. 01). CBD-DrsB1 recombinant protein had the highest inhibitory activity against Enterococcus faecalis and Bacillus subtilis, whereas DrsB1-CBD recombinant protein showed the least antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Conclusion: To the best of our knowledge, this study for the first time showed that the new recombinant peptides possess a high antibacterial activity. Acquiring resistance to antimicrobial peptides in bacteria is not readily feasible, therefore, present findings may find application as suitable alternative for current antibiotic drugs.

Cites

No record.

References

No record.

Cite

APA: Copy

Khademi, Mitra, NAZARIAN FIROUZABADI, FARHAD, & ISMAILI, AHMAD. (2019). Cloning and Expression of Two New Recombinant Antimicrobial Dermaseptin B1 Peptides in Tobacco to Control the Growth of Human Bacterial Pathogens. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 29(176), 47-60. SID. https://sid.ir/paper/361025/en

Vancouver: Copy

Khademi Mitra, NAZARIAN FIROUZABADI FARHAD, ISMAILI AHMAD. Cloning and Expression of Two New Recombinant Antimicrobial Dermaseptin B1 Peptides in Tobacco to Control the Growth of Human Bacterial Pathogens. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;29(176):47-60. Available from: https://sid.ir/paper/361025/en

IEEE: Copy

Mitra Khademi, FARHAD NAZARIAN FIROUZABADI, and AHMAD ISMAILI, “Cloning and Expression of Two New Recombinant Antimicrobial Dermaseptin B1 Peptides in Tobacco to Control the Growth of Human Bacterial Pathogens,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 29, no. 176, pp. 47–60, 2019, [Online]. Available: https://sid.ir/paper/361025/en

Related Journal Papers