EVALUATION OF CHITINASE AND β-1, 3GLUCANASE EXPRESSION PROFILE IN PSEUDOMONAS FLOURESCENS AND SALICYLIC ACID TREATED WHEAT AGAINST SEPTORIA TRITICI BLOCH (STB)

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ZAMANI ELHAM; SANJARIAN FOROUGH; MOHAMMADI GOLTAPPEH EBRAHIM; SAFAIE NASER

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Journal: Crop Biotechnology Year:2016 | Volume:5 | Issue:13 Page(s): 1-12

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Title

EVALUATION OF CHITINASE AND β-1, 3GLUCANASE EXPRESSION PROFILE IN PSEUDOMONAS FLOURESCENS AND SALICYLIC ACID TREATED WHEAT AGAINST SEPTORIA TRITICI BLOCH (STB)

Author(s)

ZAMANI ELHAM | SANJARIAN FOROUGH | MOHAMMADI GOLTAPPEH EBRAHIM | SAFAIE NASER | Issue Writer Certificate

Keywords

SALICYLIC ACIDQ1

PSEUDOMONAS FLOURESCENSQ1

β-1Q1

3 GLUCANASEQ2

CHITINASE AND SEPTORIA TRITICI BLOCH (STB)Q1

Abstract

Induced resistance is one the ways by which plants cope with the biotic stresses. Pathogenesis-related proteins (PRs), including chitinase and b-1, 3-glucanase are synthesized by plant in response to pathogenic infection. In present work, we have carried out inoculation experiment in wheat to investigate the effect of SALICYLIC ACID (SA) and PSEUDOMONAS FLOURESCENS on Septoria tritici Bloch (STB) symptoms. Subsequently, the expression profiles of chitinase and b-1, 3-glucanase were analyzed with semi-quantitative RT-PCR at 0, 4, 14 and 24 days after inoculation in both treated and control plants. Results showed that the expression of both chitinase and b-1, 3-glucanase genes increased in interaction with Z. tritici and the application of SA and P. flourescens. On the other hand, the application together had no significant effect on gene expression. Briefly, it can be concluded that increment of chitinase and β-1, 3-glucanase expression level with the application of SA and P. flourescens would play role induction of plant defense against STB.

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APA: Copy

ZAMANI, ELHAM, SANJARIAN, FOROUGH, MOHAMMADI GOLTAPPEH, EBRAHIM, & SAFAIE, NASER. (2016). EVALUATION OF CHITINASE AND β-1, 3GLUCANASE EXPRESSION PROFILE IN PSEUDOMONAS FLOURESCENS AND SALICYLIC ACID TREATED WHEAT AGAINST SEPTORIA TRITICI BLOCH (STB). JOURNAL OF CROP BIOTECHNOLOGY, 5(13), 1-12. SID. https://sid.ir/paper/226832/en

Vancouver: Copy

ZAMANI ELHAM, SANJARIAN FOROUGH, MOHAMMADI GOLTAPPEH EBRAHIM, SAFAIE NASER. EVALUATION OF CHITINASE AND β-1, 3GLUCANASE EXPRESSION PROFILE IN PSEUDOMONAS FLOURESCENS AND SALICYLIC ACID TREATED WHEAT AGAINST SEPTORIA TRITICI BLOCH (STB). JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2016;5(13):1-12. Available from: https://sid.ir/paper/226832/en

IEEE: Copy

ELHAM ZAMANI, FOROUGH SANJARIAN, EBRAHIM MOHAMMADI GOLTAPPEH, and NASER SAFAIE, “EVALUATION OF CHITINASE AND β-1, 3GLUCANASE EXPRESSION PROFILE IN PSEUDOMONAS FLOURESCENS AND SALICYLIC ACID TREATED WHEAT AGAINST SEPTORIA TRITICI BLOCH (STB),” JOURNAL OF CROP BIOTECHNOLOGY, vol. 5, no. 13, pp. 1–12, 2016, [Online]. Available: https://sid.ir/paper/226832/en

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