PROTEIN CHANGES AND ANTIOXIDANT ENZYMES ACTIVITY ON BARLEY INBRED LINES UNDER WATER SHORTAGE

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HADDAD R.; SALEK JALALI M.

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Journal: Plant Production Technology Year:2010 | Volume:9 | Issue:2 Page(s): 1-10

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Title

PROTEIN CHANGES AND ANTIOXIDANT ENZYMES ACTIVITY ON BARLEY INBRED LINES UNDER WATER SHORTAGE

Author(s)

HADDAD R. | SALEK JALALI M. | Issue Writer Certificate

Keywords

ANTIOXIDANT ENZYMESQ2

BARLEY INBRED LINESQ2

CHLOROPHYLLQ2

DROUGHTQ3

TOTAL PROTEINQ2

Abstract

So far, extensive research projects have been sort out to study effects of DROUGHT stress on the plant ANTIOXIDANT ENZYMES activity, while there is some information lacking of such studies on the field condition. In the present research project, TOTAL PROTEIN content and enzypmes activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POX) were analyzed under water shortage stress using spectrophotometer and gel assay procedures in a factorial experiment for five inbred lines of barley. Amount of TOTAL PROTEIN was significantly increased under water shortage, while amount of CHLOROPHYLL decreased. Activity of SOD and CAT, POX were significantly increased under water shortage stress. Level of enhancement in the activity of POX was more intensive for watering plants than DROUGHT stress plants. Also increased activities of POX and SOD were significant under DROUGHT stress in comparison to rain fall condition. The kinetic assays were confirmed by isozymic analysis of gel assay. Results indicated that among all studied enzymes, activity of APX was low and didn' t have important role in plant protection under water stress, while POX might have a key role as important enzyme to increase tolerance to DROUGHT stress in Hordeum vulgareinbred lines. Also, such activity of enzymes suggests that different isozymes might be induced and exhibit activity depending on water shortage stress level in barley.

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APA: Copy

HADDAD, R., & SALEK JALALI, M.. (2010). PROTEIN CHANGES AND ANTIOXIDANT ENZYMES ACTIVITY ON BARLEY INBRED LINES UNDER WATER SHORTAGE. PLANT PRODUCTS TECHNOLOGY (AGRICULTURAL RESEARCH), 9(2), 1-10. SID. https://sid.ir/paper/229736/en

Vancouver: Copy

HADDAD R., SALEK JALALI M.. PROTEIN CHANGES AND ANTIOXIDANT ENZYMES ACTIVITY ON BARLEY INBRED LINES UNDER WATER SHORTAGE. PLANT PRODUCTS TECHNOLOGY (AGRICULTURAL RESEARCH)[Internet]. 2010;9(2):1-10. Available from: https://sid.ir/paper/229736/en

IEEE: Copy

R. HADDAD, and M. SALEK JALALI, “PROTEIN CHANGES AND ANTIOXIDANT ENZYMES ACTIVITY ON BARLEY INBRED LINES UNDER WATER SHORTAGE,” PLANT PRODUCTS TECHNOLOGY (AGRICULTURAL RESEARCH), vol. 9, no. 2, pp. 1–10, 2010, [Online]. Available: https://sid.ir/paper/229736/en

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