DETECTION OF 793/B SEROTYPE OF INFECTION BRONCHITIS VIRUS FROM BROILER FLOCKS WITH RESPIRATORY SIGNS IN WEST OF MAZANDRAN PROVINCE

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HOSSEINI ALIABAD S.A.; MOMAYEZ R.; MAHMODZADEH M.; YOSEFI AMIN A.

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Journal: JOURNAL OF VETERINARY CLINICAL RESEARCH Year:2013 | Volume:4 | Issue:2 (14) Page(s): 91-97

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Title

DETECTION OF 793/B SEROTYPE OF INFECTION BRONCHITIS VIRUS FROM BROILER FLOCKS WITH RESPIRATORY SIGNS IN WEST OF MAZANDRAN PROVINCE

Author(s)

HOSSEINI ALIABAD S.A. | MOMAYEZ R. | MAHMODZADEH M. | YOSEFI AMIN A. | Issue Writer Certificate

Keywords

RESPIRATORY INFECTIONQ2

BROILER FLOCKQ2

INFECTION BRONCHITISQ2

793/B SEROTYPEQ2

NESTED RT-PCRQ1

MAZANDRANQ1

Abstract

For detection of the 793/B SEROTYPE of INFECTION BRONCHITIS virus in RESPIRATORY INFECTIONs, 11 samples were taken from BROILER FLOCKs with respiratory signs in west of MAZANDRAN province in autumn and winter of 2010. After inoculation of samples into the embryonated SPF chicken eggs, samples were checked for heamagglutination (HA) characteristic by HA test for Newcastle disease (ND) and Avian Influenza (AI) viruses, and then the cases having HA property were tested by ND and AI specific antiserum and evaluated for ND and AI virus by heamagglutination inhibition (HI) test. For identification of INFECTION BRONCHITIS virus, 1 ml of allantoic fluid was harvested from allantoic fluid and viral RNA was extracted with RNA purification kit and at the first step a fragment of S1 gene was amplified by general primers of IB virus in RT-PCR reaction. In the second step the RT-PCR product was amplified for differentiation of Mass and 793/B by type specific primers in Nested-PCR. Results revealed that 7 of 11 samples were positive for IB virus in RT-PCR reaction and 5 samples were positive for 793/B SEROTYPE in Nested-PCR. While the affected flocks didn’t take any vaccination against 793/B SEROTYPE, samples were considered positive for 793/B SEROTYPE infection. Also, ND virus and AI virus were detected in two IB positive samples.

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APA: Copy

HOSSEINI ALIABAD, S.A., MOMAYEZ, R., MAHMODZADEH, M., & YOSEFI AMIN, A.. (2013). DETECTION OF 793/B SEROTYPE OF INFECTION BRONCHITIS VIRUS FROM BROILER FLOCKS WITH RESPIRATORY SIGNS IN WEST OF MAZANDRAN PROVINCE. JOURNAL OF VETERINARY CLINICAL RESEARCH, 4(2 (14)), 91-97. SID. https://sid.ir/paper/233018/en

Vancouver: Copy

HOSSEINI ALIABAD S.A., MOMAYEZ R., MAHMODZADEH M., YOSEFI AMIN A.. DETECTION OF 793/B SEROTYPE OF INFECTION BRONCHITIS VIRUS FROM BROILER FLOCKS WITH RESPIRATORY SIGNS IN WEST OF MAZANDRAN PROVINCE. JOURNAL OF VETERINARY CLINICAL RESEARCH[Internet]. 2013;4(2 (14)):91-97. Available from: https://sid.ir/paper/233018/en

IEEE: Copy

S.A. HOSSEINI ALIABAD, R. MOMAYEZ, M. MAHMODZADEH, and A. YOSEFI AMIN, “DETECTION OF 793/B SEROTYPE OF INFECTION BRONCHITIS VIRUS FROM BROILER FLOCKS WITH RESPIRATORY SIGNS IN WEST OF MAZANDRAN PROVINCE,” JOURNAL OF VETERINARY CLINICAL RESEARCH, vol. 4, no. 2 (14), pp. 91–97, 2013, [Online]. Available: https://sid.ir/paper/233018/en

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