INVESTIGATION OF ANTIBACTERIAL EFFECTS OF CELL SUSPENSION CULTURE AND COMPARISON BY ESSENTIAL OILS AND SEED EXTRACT IN BUNIUM PERSICUM

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KHOSRAVINIA S.; ZIARATNIA S.M.; BAGHERI A.; MARASHI S.H.

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Journal: Research and Innovation in Food Science and Technology Year:2013 | Volume:2 | Issue:1 Page(s): 79-92

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Title

INVESTIGATION OF ANTIBACTERIAL EFFECTS OF CELL SUSPENSION CULTURE AND COMPARISON BY ESSENTIAL OILS AND SEED EXTRACT IN BUNIUM PERSICUM

Author(s)

KHOSRAVINIA S. | ZIARATNIA S.M. | BAGHERI A. | MARASHI S.H. | Issue Writer Certificate

Keywords

ANTIBACTERIAL EFFECTQ1

BUNIUM PERSICUMQ1

CELL SUSPENSION CULTUREQ2

ESSENTIAL OILSQ2

EXTRACTQ1

Abstract

Recently by no incident there is a great concern in people to consume herbal antibiotics rather than using chemical ones due to bacterial resistances witnessed in chemical treatments. In this research the antibacterial activity of aqueous and ethanol EXTRACTs of black zira CELL SUSPENSION CULTURE in comparison with seed EXTRACTs and ESSENTIAL OILS were investigated against Staphylococus aurous, Escherichia coliand Bacillus subtilis using disk diffusion and digging hole methods. The bacterial concentration was at 106 and 107 CFU/mL. Positive and negative controls were antibiotics (Gentamicin [10 mg/disk], Tetracycline [30 mg/disk]) and solvents (5% DMSO and 80% Ethanol) respectively. Inhibitory effects were studied by measuring the growth inhibiting circle diameter. The Results showed that the range of Minimum Inhibitory Concentration (MIC) were 3.12-6.25, 12.5-50 and 25-50 mg/ml, for the essential oil, the cell EXTRACT and the seed EXTRACT respectively. According to the results a promising antibacterial activities were revealed from all black zira samples against tested bacteria with the maximum activity to the S. aurous. In contrast to E. coli, the effect of EXTRACTs on S. aurous and B. subtilis was dependent to the bacteria concentration. Overall ANTIBACTERIAL EFFECTs of cell EXTRACTs against tested bacteria were lesser than ESSENTIAL OILS (100%) and higher than seed EXTRACTs and different dilution of ESSENTIAL OILS, therefore it seems CELL SUSPENSION CULTURE of black zira is a suitable method to produce antibacterial compounds.

Cites

INFLUENCE OF DIFFERENT CONCENTRATIONS OF ETHANOLIC EXTRACT OF SEVEN MEDICINAL PLANTS ON THREE BACTERIA STRAINS

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APA: Copy

KHOSRAVINIA, S., ZIARATNIA, S.M., BAGHERI, A., & MARASHI, S.H.. (2013). INVESTIGATION OF ANTIBACTERIAL EFFECTS OF CELL SUSPENSION CULTURE AND COMPARISON BY ESSENTIAL OILS AND SEED EXTRACT IN BUNIUM PERSICUM. JOURNAL OF RESEARCH AND INNOVATION IN FOOD SCIENCE AND TECHNOLOGY, 2(1), 79-92. SID. https://sid.ir/paper/234021/en

Vancouver: Copy

KHOSRAVINIA S., ZIARATNIA S.M., BAGHERI A., MARASHI S.H.. INVESTIGATION OF ANTIBACTERIAL EFFECTS OF CELL SUSPENSION CULTURE AND COMPARISON BY ESSENTIAL OILS AND SEED EXTRACT IN BUNIUM PERSICUM. JOURNAL OF RESEARCH AND INNOVATION IN FOOD SCIENCE AND TECHNOLOGY[Internet]. 2013;2(1):79-92. Available from: https://sid.ir/paper/234021/en

IEEE: Copy

S. KHOSRAVINIA, S.M. ZIARATNIA, A. BAGHERI, and S.H. MARASHI, “INVESTIGATION OF ANTIBACTERIAL EFFECTS OF CELL SUSPENSION CULTURE AND COMPARISON BY ESSENTIAL OILS AND SEED EXTRACT IN BUNIUM PERSICUM,” JOURNAL OF RESEARCH AND INNOVATION IN FOOD SCIENCE AND TECHNOLOGY, vol. 2, no. 1, pp. 79–92, 2013, [Online]. Available: https://sid.ir/paper/234021/en

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