Development and molecular analyses of Xanthomonas pthA specific scFv recombinant monoclonal antibodies

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RAEISI HAMIDEH; SAFARNEJAD MOHAMMAD REZA; ALAVI SEYED MEHDI; FARROKHI NASER; ELAHINIA SEYED ALI; SAFARPOUR HOSSEIN; sharifian farshid

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF CROP PROTECTION Year:2019 | Volume:8 | Issue:4 Page(s): 417-429

Download Full-Text

Persian Verion

View:

305

Download:

137

Cites:

Information Journal Paper

Title

Development and molecular analyses of Xanthomonas pthA specific scFv recombinant monoclonal antibodies

Author(s)

Keywords

BiopanningQ1

citrus bacterial cankerQ1

phage displayQ1

single chain fragment variableQ1

Abstract

The Xanthomonas citri pv. citri (Xcc) is causal agent of bacterial citrus canker which is major disease of citrus throughout the world. The pthA bacterial effector protein is presented within the infected plants and indispensable of canker. The scFv antibodies are valuable tools for diagnosis and suppression of pathogens within plants. The present article describes developing and characterization of specific recombinant monoclonal scFv antibodies against pthA effector protein. For this aim, the gene encoding pthA protein was heterologously expressed in Escherichia coli and used for screening of Tomlinson phage display antibody library to pinpoint specific single chain variable fragment (scFv). In each round of panning, the affinity of phage towards pthA was checked by enzyme linked immunosorbent assay (ELISA). The data was indicative of about 50% of the monoclonal phages to be reactive strongly against pthA protein. Among the positive clones, 5 samples (A12, B8, C1, H8 and G8) were capable of detecting Xcc-infected plant samples and recombinant pthA protein. Restriction fragment length polymorphism showed similar banding pattern for all 5 scFvs as renamed to pthA-scFG8. HB2151 E. coli cells were infected by the phage bearing pthAscFG8, and the expression of the peptide was induced by IPTG to produce a 30 kDa recombinant molecule. I-TASSER was used for homology modeling of both scFv and pthA and docking was carried out by Hex program. The latter demonstrated binding energy of − 784 kcal/mol in scFv-pthA.

Cites

No record.

References

No record.

Cite

APA: Copy

RAEISI, HAMIDEH, SAFARNEJAD, MOHAMMAD REZA, ALAVI, SEYED MEHDI, FARROKHI, NASER, ELAHINIA, SEYED ALI, SAFARPOUR, HOSSEIN, & sharifian, farshid. (2019). Development and molecular analyses of Xanthomonas pthA specific scFv recombinant monoclonal antibodies. JOURNAL OF CROP PROTECTION, 8(4 ), 417-429. SID. https://sid.ir/paper/238919/en

Vancouver: Copy

RAEISI HAMIDEH, SAFARNEJAD MOHAMMAD REZA, ALAVI SEYED MEHDI, FARROKHI NASER, ELAHINIA SEYED ALI, SAFARPOUR HOSSEIN, sharifian farshid. Development and molecular analyses of Xanthomonas pthA specific scFv recombinant monoclonal antibodies. JOURNAL OF CROP PROTECTION[Internet]. 2019;8(4 ):417-429. Available from: https://sid.ir/paper/238919/en

IEEE: Copy

HAMIDEH RAEISI, MOHAMMAD REZA SAFARNEJAD, SEYED MEHDI ALAVI, NASER FARROKHI, SEYED ALI ELAHINIA, HOSSEIN SAFARPOUR, and farshid sharifian, “Development and molecular analyses of Xanthomonas pthA specific scFv recombinant monoclonal antibodies,” JOURNAL OF CROP PROTECTION, vol. 8, no. 4 , pp. 417–429, 2019, [Online]. Available: https://sid.ir/paper/238919/en

Related Journal Papers