SCHINUS TEREBINTHIFOLIUS RADDI (ANACARDIACEAE) LEAF EXTRACTS: ANTIBACTERIAL ACTIVITY AGAINST TWO AGROBACTERIUM TUMEFACIENS STRAINS

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GHANNEY NADIA; RHOUMA ALI

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Journal: JOURNAL OF CROP PROTECTION Year:2015 | Volume:4 | Issue:1 Page(s): 85-96

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Title

SCHINUS TEREBINTHIFOLIUS RADDI (ANACARDIACEAE) LEAF EXTRACTS: ANTIBACTERIAL ACTIVITY AGAINST TWO AGROBACTERIUM TUMEFACIENS STRAINS

Author(s)

GHANNEY NADIA | RHOUMA ALI | Issue Writer Certificate

Keywords

BRAZILIAN PEPPERTREEQ2

LEAF EXTRACTSQ2

THE CROWN GALL DISEASEQ2

ANTIBACTERIAL ACTIVITYQ2

Abstract

BRAZILIAN PEPPERTREE (Schinus terebinthifolius) leaf extract was applied against two strains of THE CROWN GALL DISEASE (Agrobacterium tumefaciens); strains C58 and AR125. In vitro, we used the agar well diffusion method and the extract was tested in different solvents selected according to their polarity indices, at different concentrations and different incubation temperatures. In vivo, we tested the extract prepared in different solvents on the stems of young tomato plants (cv. Firenze) which were inoculated with the strain C58.The best results were obtained with the extract prepared in hot sterile distilled water and in methanol (0.3 g.ml-1) incubated at 25 oC and 30 oC. The minimum inhibitory concentration (MIC) was evaluated to 10-4 g.ml-1. In another aspect, to identify the nature of certain substances from S. terebinthifolius LEAF EXTRACTS, we made a test of fractionation using the Thin Layer Chromatography (TLC) method and phytochemical screening of the crude methanol leaf extract. We noticed the presence of alkaloids and flavonoid compounds which may be responsible for the ANTIBACTERIAL ACTIVITY. These tests indicated that false pepper leaf extract has an antibiotic effect against Agrobacterium tumefaciens both in vitro and in vivo, which represents a conceptual approach with great promise for future biological control.

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APA: Copy

GHANNEY, NADIA, & RHOUMA, ALI. (2015). SCHINUS TEREBINTHIFOLIUS RADDI (ANACARDIACEAE) LEAF EXTRACTS: ANTIBACTERIAL ACTIVITY AGAINST TWO AGROBACTERIUM TUMEFACIENS STRAINS. JOURNAL OF CROP PROTECTION, 4(1), 85-96. SID. https://sid.ir/paper/238955/en

Vancouver: Copy

GHANNEY NADIA, RHOUMA ALI. SCHINUS TEREBINTHIFOLIUS RADDI (ANACARDIACEAE) LEAF EXTRACTS: ANTIBACTERIAL ACTIVITY AGAINST TWO AGROBACTERIUM TUMEFACIENS STRAINS. JOURNAL OF CROP PROTECTION[Internet]. 2015;4(1):85-96. Available from: https://sid.ir/paper/238955/en

IEEE: Copy

NADIA GHANNEY, and ALI RHOUMA, “SCHINUS TEREBINTHIFOLIUS RADDI (ANACARDIACEAE) LEAF EXTRACTS: ANTIBACTERIAL ACTIVITY AGAINST TWO AGROBACTERIUM TUMEFACIENS STRAINS,” JOURNAL OF CROP PROTECTION, vol. 4, no. 1, pp. 85–96, 2015, [Online]. Available: https://sid.ir/paper/238955/en

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