Biochemical characterization of digestive carbohydrases of the tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae) larvae in response to feeding on six tomato cultivars

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Sadeghinasab Fereshteh; SAFAVI SEYED ALI; GHADAMYARI MOHAMMAD; HOSSEININAVEH VAHID

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Journal: JOURNAL OF CROP PROTECTION Year:2017 | Volume:6 | Issue:3 Page(s): 377-389

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Information Journal Paper

Title

Biochemical characterization of digestive carbohydrases of the tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae) larvae in response to feeding on six tomato cultivars

Author(s)

Sadeghinasab Fereshteh | SAFAVI SEYED ALI | GHADAMYARI MOHAMMAD | HOSSEININAVEH VAHID | Issue Writer Certificate

Keywords

Tuta absolutaQ1

αQ1

-amylaseQ1

αQ1

/ßQ1

-glycosidaseQ1

αQ1

/ßQ1

-galactosidaseQ1

Abstract

The tomato leaf miner, Tuta absoluta (Meyrick) is an imported pest and serious threat to tomato production in farms and greenhouses of Iran. Use of genetically engineered plants expressing carbohydrase inhibitors is one of the non-chemical methods for controlling insect pests, and knowledge about enzymatic properties of carbohydrases will help us to achieve this goal. Therefore, in present study we characterized biochemical properties of digestive carbohydrases in the midgut of last larval instar of T. absoluta fed on different tomato cultivars (Kingston, Riogrande, Super Luna, Super Chief, Super strain B and Calj). While the highest amylolytic activity was on Super strain B, the lowest was on Super Chief. The optimal pH and temperature for α;-amylase were found to be at pH 9. 0 and 45 ° C, respectively. As calculated from Lineweaver-Burk plots, the highest Km and Vmax values for α;-amylase obtained in Super Chief and Super Luna cultivars were 0. 565 ± 0. 11mM and 2. 287 ± 0. 4mM/min, respectively. The effects of different compounds on amylolytic activity indicated that CaCl2, MgCl2, NaCl and KCl increased amylase activity, whereas EDTA, ZnCl2 and BaCl2 decreased the enzyme activity in Super Luna cultivar. The highest activity of α;-/ß;-glucosidases was observed at pH 6. 0 and 7. 0, respectively, whereas the optimal pH for α; /ß;-galactosidases was at 5. 0. The highest specific activity of α;-/ß;-glucosidases was determined in Riogrande-fed larvae, whereas the highest α; /ß;-galactosidases activity was in the larvae fed on Riogrande and Calj cultivars, respectively. By the native-PAGE, two bands were clearly detected for α;-amylase. Since the larvae reared on Kingston showed lowest carbohydrase activities, this cultivar could possibly be suggested as the least suitable host for feeding of T. absoluta.

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APA: Copy

Sadeghinasab, Fereshteh, SAFAVI, SEYED ALI, GHADAMYARI, MOHAMMAD, & HOSSEININAVEH, VAHID. (2017). Biochemical characterization of digestive carbohydrases of the tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae) larvae in response to feeding on six tomato cultivars. JOURNAL OF CROP PROTECTION, 6(3 ), 377-389. SID. https://sid.ir/paper/238976/en

Vancouver: Copy

Sadeghinasab Fereshteh, SAFAVI SEYED ALI, GHADAMYARI MOHAMMAD, HOSSEININAVEH VAHID. Biochemical characterization of digestive carbohydrases of the tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae) larvae in response to feeding on six tomato cultivars. JOURNAL OF CROP PROTECTION[Internet]. 2017;6(3 ):377-389. Available from: https://sid.ir/paper/238976/en

IEEE: Copy

Fereshteh Sadeghinasab, SEYED ALI SAFAVI, MOHAMMAD GHADAMYARI, and VAHID HOSSEININAVEH, “Biochemical characterization of digestive carbohydrases of the tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae) larvae in response to feeding on six tomato cultivars,” JOURNAL OF CROP PROTECTION, vol. 6, no. 3 , pp. 377–389, 2017, [Online]. Available: https://sid.ir/paper/238976/en

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